A method for detecting plasma active components and a plasma card using the method

Abstract

The invention discloses a method for detecting active components in plasma and a plasma card applying the method, and relates to methods for detecting active components such as proteins, polypeptides, free DNA (deoxyribonucleic acid), small molecule metabolites, inorganic ions, medicines and toxicants in plasma. Real-time separation and quantitative collection are carried out on the plasma in whole-blood samples by the aid of trace plasma preparation equipment, and the plasma is used as a detected material. Blood cell separation layers of the trace plasma preparation equipment are made of microporous polypropylene materials, blood cells in whole-blood are separated and filtered by the aid of volume exclusion technical principles, and plasma adsorption layers are made of cellulose materials. According to the method and the plasma card, simple and speedy preparation methods with functions of quantitatively collecting the plasma are provided, and the active components in the plasma can be solidified, keep stable and can be detected by chemical processes, physical processes, chromatography processes, mass spectrometry processes, biochemical processes, biological processes, immunization processes and gene sequencing processes.

Description

technical field [0001] The invention relates to the technical field of medical detection, in particular to a detection method and equipment for plasma active components. Background technique [0002] The conventional blood collection method needs to use a syringe to puncture the vein first, draw a few milliliters to tens of milliliters of peripheral blood into a blood collection tube, transport it to a testing laboratory, centrifuge to remove blood cells, and then use various methods for testing. The trauma of blood sample collection is obvious, the blood collection volume is large, and the transportation time limit has a great impact on the stability of active ingredients. Additional equipment such as blood collection tubes and centrifuges are required, and it is difficult to carry out sample collection in places other than specialized blood collection institutions such as medical and health systems. In addition, the active components of the whole blood that has not been ce...

AI Technical Summary

Problems solved by technology

However, DBS requires a special puncher, which is difficult to achieve quantitative collection. In addition, it also has the problem that it cannot be centrifuged in time. The blood volume is easily affected by the hematocrit, and blood cells cannot be removed. Hemolysis of red blood cells in whole blood may affect the detection of active ingredients. Potential interference, can only be used for qualitative or quantitative detection purposes

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