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Thin layer chromatographic analysis method for detection of flavonoids in polygonum viviparum

A technique of thin-layer chromatography and analysis method, which is applied in the field of thin-layer chromatography for detecting flavonoids in Polygonum vulgaris, can solve the problems of high technical requirements for experimenters, high dosage of toxic reagents, and high instrument cost, and achieves strong specificity. , The effect of authenticity identification and quality control is simple and accurate

Active Publication Date: 2017-03-22
YICHANG SHANCHENGSHUIDU CORDYCEPS
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AI Technical Summary

Problems solved by technology

The above HPLC analysis method has high instrument cost, complicated operation, high skill requirements for experimenters, and a large amount of toxic reagents. At the same time, only a single sample can be tested at a time.

Method used

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  • Thin layer chromatographic analysis method for detection of flavonoids in polygonum viviparum
  • Thin layer chromatographic analysis method for detection of flavonoids in polygonum viviparum
  • Thin layer chromatographic analysis method for detection of flavonoids in polygonum viviparum

Examples

Experimental program
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Effect test

Embodiment 1

[0027] Preparation of the test solution: Take 10 g of the sample and place it in a 500 mL round-bottomed flask, add 250 mL of anhydrous methanol, then add 60 mL of dilute hydrochloric acid with a mass fraction of 25%-30%, reflux in a water bath at 80°C for 2 hours, and place it on ice immediately after taking it out Cool in a bath, filter, take the filtrate and evaporate to dryness under reduced pressure on a rotary evaporator to remove the solvent, add 1 mL of methanol to the residue to dissolve, centrifuge to get the supernatant, mix the sample with 2 g of 200-300 mesh silica gel, and set aside.

[0028] Weigh 25g of 200-300 mesh silica gel, stir it with dichloromethane until there are no bubbles, pack it into a column with a volume of 70mL, use dichloromethane:methanol=100:5 to elute, and collect the polar segment A, Evaporate the eluents of polar segment B and polar segment C to dryness, and add 2 mL of methanol to redissolve, respectively, to obtain 3 parts of the test sol...

Embodiment 2

[0033] Different from Example 1, the conditions of thin-layer analysis are as follows, take each 10 μL of the test solution and the reference solution, and place it at 10 mm from the bottom edge of the silica gel G thin-layer plate, with a volume ratio of 25:0.6:0.5: 2 The dichloromethane-acetone-methanol-formic acid system is used as a developing agent, and the above-mentioned developing system is pre-balanced for 10 minutes on one side of the double-slot developing cylinder under the condition of a temperature of 22°C and a humidity of 70%. 10cm, carry out secondary expansion. The condition of color development is to spray 5% AlCl on the volatilized thin-layer plate 3 ,, dry it, put it under ultraviolet light to inspect the fluorescent TLC spots, and obtain the flavonoid composition map of Polygonum versicolor medicinal material, see figure 2 . 1, 2, 3, and 4 in the spectrum are the substances of the polar segment A of the test product, the reference substance of isorhamn...

Embodiment 3

[0035] The difference from Example 1 is that the conditions of TLC analysis are as follows, take 10 μL each of the test solution and the reference solution, and place it at a distance of 10 mm from the silica gel G thin-layer plate, and use a volume ratio of 30:1.0:0.5:2 The dichloromethane-acetone-methanol-formic acid system is used as a developing agent. Under the conditions of temperature 25°C and humidity 80%, add the above-mentioned expansion system to one side of the double-slot expansion cylinder to pre-balance for 10 minutes, and expand upward with a expansion distance of 10cm. , for a second expansion. The condition for color development is to spray 7% AlCl on the volatilized thin-layer plate 3 The solution was dried in the air, and the fluorescent thin-layer chromatography was inspected under an ultraviolet lamp to obtain the flavonoid component spectrum of the Polygonum versicolor medicinal material, see image 3 . 1, 2, 3, and 4 in the spectrum are the substances...

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Abstract

The invention relates to a thin layer chromatographic analysis method for detection of flavonoids in polygonum viviparum. The method comprises the following steps: (a) preparation of a test sample solution: carrying out hydrolysis extraction of a polygonum viviparum medicinal material with hydrochloric acid methanol, to be used as the polygonum polygonum test sample solution; (b) preparation of reference substance solutions: respectively dissolving three reference substances of quercetin, isorhamnetin and kaempferol by methanol, to obtain the reference substance solutions; and (c) thin layer chromatography detection: with use of a silica gel thin layer chromatography plate, spreading the test sample and the reference substance solutions with a dichloromethane-acetone-methanol-formic acid system as a spreading agent; and spraying an AlCl3 chromogenic solution, and carrying out visual inspection of fluorescent thin layer chromatographic spots under an ultraviolet lamp. The method has the advantages of high efficiency, low cost and high practical value, and provides an effective way for identification and quality evaluation of the polygonum viviparum medicinal materials.

Description

technical field [0001] The invention relates to the field of quality detection of traditional Chinese medicinal materials, in particular to a thin-layer chromatographic analysis method for detecting flavonoid components in Polygonum versicolor. [0002] technical background [0003] This product is the rhizome of Polygonum viviparum L., a plant of the Polygonaceae family. It is recorded in the "Tibetan Medicine Standards Issued by the Ministry of Health Volume I", and has the effects of relieving diarrhea and invigorating the stomach. Polygonum victoriae mainly grows in hillside grasslands, valley streams and other places at an altitude of 1500-5000m. It is widely distributed in Tibet, Qinghai, Gansu and other places. It is known as "plateau boxing ginseng". components such as protein and polysaccharides. Among them, flavonoids are the main secondary metabolites of Polygonum viburnum, which have strong anti-free radical effect and antioxidant capacity. It has been reported t...

Claims

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Application Information

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IPC IPC(8): G01N30/90G01N30/94
CPCG01N30/90G01N30/94
Inventor 孙益阔李文庆廖娜甄达明钱正明李文佳华献春
Owner YICHANG SHANCHENGSHUIDU CORDYCEPS
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