Method for increasing seedling rate and seedling survival rate of endangered plant Tibetan paeonia ludlowii

A technology of big flower yellow and seedling rate, applied in botany equipment and methods, plant regeneration, horticultural methods, etc., can solve the problems of long seedling time, low seedling survival rate of Tibetan big flower yellow peony seedlings, etc., to achieve Effects of increasing germination rate, shortening seedling raising period and increasing population quantity

Inactive Publication Date: 2017-03-29
TIBET AGRI & ANIMAL HUSBANDRY COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] The present invention aims to solve the problems of low seedling rate and seedling survival rate of the endangered horticultural plant Tibetan

Method used

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  • Method for increasing seedling rate and seedling survival rate of endangered plant Tibetan paeonia ludlowii
  • Method for increasing seedling rate and seedling survival rate of endangered plant Tibetan paeonia ludlowii
  • Method for increasing seedling rate and seedling survival rate of endangered plant Tibetan paeonia ludlowii

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Experimental program
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specific Embodiment approach 1

[0039] Specific implementation mode one: a kind of method for improving the seedling rate and seedling survival rate of the endangered plant Tibetan big flower peony comprises the following steps:

[0040] Step 1: Disinfect the seeds of the collected big-flowered peony;

[0041] Step 2: preparing MS medium mother liquor;

[0042] Step 3: preparing a culture medium for inducing buds of peony grandiflora;

[0043]The specific process is: add 6-BA with a concentration of 0.5-1mg / L, IAA with a concentration of 0.3-0.5mg / L, and GA with a concentration of 0.2-0.5mg / L in the MS medium mother solution prepared in step 2. 3 ; The 6-BA is 6-benzyl adenine, and IAA is indole acetic acid, GA 3 is gibberellin;

[0044] Step 4: Peel off the seed coat and endosperm of the sterilized Paeonia grandiflora seeds in step 1, and after separating the seed embryos, inoculate the seed embryos onto the surface of the Paeonia grandiflora bud induction medium prepared in step 3; peel off the seed emb...

specific Embodiment approach 2

[0049] Specific embodiment two: the difference between this embodiment and specific embodiment one is that: the specific process of disinfecting the seeds of the collected peony grandiflora in said step one is:

[0050] Add 75% ethanol to the collected seeds (use absolute ethanol to configure 75% ethanol by volume), disinfect for 30-35s, rinse with sterile water 1-2 times, add 0.1% mercuric chloride (mass percentage), Treat for 15min-16min, rinse with sterile water 2-3 times, add 60-65mL sterile water to soak for 2-3 days.

[0051] Other steps and parameters are the same as those in Embodiment 1.

specific Embodiment approach 3

[0052] Specific embodiment three: the difference between this embodiment and specific embodiment one or two is: the specific process of preparing MS culture medium mother liquor in described step 2 is:

[0053] Take 100mL of macroelements, 10mL of trace elements, 10mL of iron salts, and 10mL of organic matter, add 30g of sucrose, add distilled water to 1L, adjust the pH to 6.0, add 7g of agar powder, and autoclave at 121°C for 15 minutes.

[0054] Table 1 MS basal medium mother solution configuration table

[0055]

[0056]

[0057] MS medium was designed by Murashige and Skoog for tobacco cell culture in 1962. It is characterized by high concentration of inorganic salts and ions. It is a relatively stable ion balance solution. It has high nitrate content and the quantity and ratio of nutrients are appropriate. It can meet the nutritional and physiological needs of plant cells, so it has a wide range of applications. It is used as the basic medium for the rapid propagati...

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Abstract

The invention discloses a method for increasing the seedling rate and seedling survival rate of the endangered plant Tibetan paeonia ludlowii and relates to a method for increasing the seedling rate and seedling survival rate of Tibetan paeonia ludlowii. The invention aims at solving the problems of low seedling rate and seedling survival rate and long seedling culture time of the endangered horticultural plant Tibetan paeonia ludlowii. The method comprises the following steps: (I) disinfecting the collected seeds of paeonia ludlowii; (II) preparing MS medium mother liquid; (III) preparing a paeonia ludlowii induction medium; (IV) stripping the peel and endosperm of the paeonia ludlowii seeds, separating out the embryo and inoculating to the surface of the paeonia ludlowii induction medium; (V) culturing in an incubator to obtain aseptic seedlings; (VI) preparing a rooting medium; (VII) inoculating the aseptic seedlings into the rooting medium for rooting culture; and (VIII) acclimatizing and transplanting the aseptic seedlings after the rooting culture. The method disclosed by the invention is applied to the field of Tibetan endangered plant resource protection.

Description

technical field [0001] The invention relates to a method for improving the seedling emergence rate and seedling survival rate of the endangered plant Tibetan big flower peony. Background technique [0002] Paeonia ludlowii (Stern et Taylor) Hong is a species endemic to Tibet. It is a clustered shrub. It is a national second-class endangered plant. There are about 6,000 wild plants. Weak, highly dependent on water, common in roadsides, mountainsides, creeks and rivers. Distributed in an open valley with a length of about 100km from Bayi Town to Milin County in Nyingchi Prefecture, Tibet, with a vertical distribution range of 2500-3500m. [0003] 1. Factors that lead to the endangerment of the big yellow peony [0004] 1.1 Physiological characteristics and environmental factors [0005] The wild yellow peony has a narrow distribution range, a small number, and the population is declining. There are many reasons for this; physiological characteristics, special growth environ...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 禄亚洲尹秀李芳兰小中
Owner TIBET AGRI & ANIMAL HUSBANDRY COLLEGE
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