Method for separating and purifying flavonoid glycoside compounds in lotus plumules through high-speed countercurrent chromatography

A high-speed counter-current chromatography, separation and purification technology is applied in the field of high-speed counter-current chromatography to separate and purify flavonoid glycosides in lotus seeds, which can solve the problems of large solvent consumption, large sample loss, complicated operation, etc., and achieves low comprehensive cost and small sample loss. , the effect of simple operation

Active Publication Date: 2017-03-29
INST OF APPLIED CHEM JIANGXI ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

); in the prior art, methods such as traditional column chromatography separation and recrystallization are generally used to extract and separate monomeric compounds from the lotus seed heart sample. Thes

Method used

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  • Method for separating and purifying flavonoid glycoside compounds in lotus plumules through high-speed countercurrent chromatography
  • Method for separating and purifying flavonoid glycoside compounds in lotus plumules through high-speed countercurrent chromatography
  • Method for separating and purifying flavonoid glycoside compounds in lotus plumules through high-speed countercurrent chromatography

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Step 1: Preparation of lotus seed heart ethanol extract

[0033] Take 1.0 kg of lotus seed heart that was naturally dried in the shade, crush it, and perform ultrasonic extraction with a concentration of 80% ethanol as a solvent, the solid-to-liquid ratio is 1:20, the extraction time is 2 hours, filter, and the filter residue is treated twice; the filtrates are combined and concentrated under reduced pressure Until there is no ethanol smell, the ethanol extract of lotus seed heart is obtained.

[0034] Step 2: Preparation of crude flavonoids from lotus seed heart

[0035] Filter the ethanol extract of lotus seed heart, select macroporous resin, polyamide, silica gel, reverse silica gel or gel as the adsorption material to absorb and filter the ethanol extract of lotus seed heart, then first use water and then use 10% to 90% ethanol Gradient elution; specifically: first elution with 10 times column volume of water, and then elution with 10%, 30%, 50%, 70%, 90% ethanol o...

Embodiment 2

[0044] Step 1: Preparation of lotus seed heart ethanol extract

[0045] Take 1.0 kg of lotus seed heart that was naturally dried in the shade, crush it, and perform ultrasonic extraction with a concentration of 70% ethanol as a solvent, the solid-to-liquid ratio is 1:30, the extraction time is 1 hour, filter, and repeat the treatment of the filter residue once; combine the filtrates and concentrate under reduced pressure Until there is no ethanol smell, the crude ethanol extract of lotus seed heart is obtained.

[0046] Step 2: Preparation of crude flavonoids from lotus seed heart

[0047] Filter the lotus seed heart ethanol extract, select macroporous resin, polyamide, silica gel, reverse silica gel or gel as the adsorption material to absorb and filter the lotus seed heart crude extract, then use water and then use 10% to 90% ethanol Gradient elution; specifically: first elution with 10 times column volume of water, and then elution with 10%, 30%, 50%, 70%, 90% ethanol of 5...

Embodiment 3

[0053] Step 1: Preparation of lotus seed heart ethanol extract

[0054] Take 1.0 kg of lotus seed heart that was naturally dried in the shade, crush it, and perform ultrasonic extraction with a concentration of 90% ethanol as a solvent. The solid-to-liquid ratio is 1:10, and the extraction time is 3 hours. Filter and repeat the treatment of the filter residue twice; combine the filtrates and concentrate under reduced pressure Until there is no ethanol smell, the ethanol extract of lotus seed heart is obtained.

[0055] Step 2: Preparation of crude flavonoids from lotus seed heart

[0056] Filter the ethanol extract of lotus seed heart, select macroporous resin, polyamide, silica gel, reverse silica gel or gel as the adsorption material to absorb and filter the ethanol extract of lotus seed heart, then first use water and then use 10% to 90% ethanol Gradient elution; specifically: first elution with 10 times column volume of water, and then elution with 10%, 30%, 50%, 70%, 90%...

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Abstract

The invention provides a method for separating and purifying flavonoid glycoside compounds in lotus plumules. The method comprises the following steps of 1 preparing of a lotus plumule solution, 2 enrichment of lotus plumule total flavonoids and 3 separating and purifying through high-speed countercurrent chromatography, and quercetin-3-O-beta-D-glucoside, isorhanetin-3-O-beta-D-glucoside, apigenin-6-C-beta-D-xylose-8-C-beta-D-glucoside and apigenin-6-C-beta-D-glucose-8-C-beta-D-glucoside are obtained. The flavonoid glycoside compound monomers separated and purified through the method are high in purity, low in loss, easy to operate, high in preparation quantity, economical and environmentally friendly.

Description

technical field [0001] The invention relates to the field of natural medicinal chemistry, in particular to a method for separating and purifying flavonoid glycosides from lotus seeds by high-speed countercurrent chromatography. Background technique [0002] Lianzixin is the radicle and young leaves of the mature seeds of the water lily family aquatic herbaceous plant lotus (Nelumbonucifera Gaertn). It is a variety recorded in the "Chinese Pharmacopoeia" in previous editions. It has pharmacological properties such as lowering blood pressure, lowering blood sugar, antiarrhythmia, anti-oxidation, anti-myocardial ischemia, relaxing smooth muscle, strengthening the heart, anti-cancer, protecting cerebral ischemia injury, improving acute lung injury and pulmonary fibrosis, central nervous system inhibition, and lowering blood sugar. effect. Modern research shows that lotus seed heart mainly contains active ingredients such as alkaloids, flavonoids and polysaccharides. A large num...

Claims

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Application Information

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IPC IPC(8): C07H17/07C07H1/08C07D407/14
CPCC07D407/14C07H1/08C07H17/07
Inventor 吴磊熊伟胡居吾韩晓丹付建平
Owner INST OF APPLIED CHEM JIANGXI ACAD OF SCI
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