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A kind of preparation method of graphene glass chip

A glass chip and graphene technology, which is applied in the field of refractive index sensing and biomolecular detection, can solve the problems of weak interaction between graphene and substrate, difficulty in precisely controlling the thickness of graphene, and easy washing of graphene layers. , to achieve the effect of strong repeatability, strong binding force and long service life

Active Publication Date: 2018-10-19
北京石墨烯研究院有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, using the conventional transfer method to transfer the graphene on the metal substrate to the surface of the glass substrate, since the chip product requires a large number of graphene layers, many times of repeated transfer are required, the process is very complicated, and the cost is inevitably high. And the transferred graphene has weak interaction with the substrate, the graphene layer is easily washed off, and the service life of the chip is short; the graphene glass chip prepared by the method of reducing graphene oxide is difficult to accurately control the thickness of graphene, each chip The thickness of the graphene layer varies, resulting in inconsistent measurement results each time, which is fatal for commercial testing instruments

Method used

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  • A kind of preparation method of graphene glass chip
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  • A kind of preparation method of graphene glass chip

Examples

Experimental program
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Effect test

Embodiment 1

[0039] Embodiment 1: the preparation of graphene glass chip

[0040] On the cleaned quartz glass substrate, a graphene film with a controllable layer number is directly grown by chemical vapor deposition technology, and various biomolecules are coupled on the surface of the graphene, so as to obtain such a figure 1 The application shown is a graphene-on-glass chip for detecting interactions between biomolecules. The specific method includes the following steps:

[0041] 1) Quartz glass substrate cleaning:

[0042] Use acetone, isopropanol, and ultrapure water to ultrasonically clean a quartz glass substrate with a size of 15mm×15mm×1mm for 10 minutes, and dry it with nitrogen;

[0043] 2) Graphene growth:

[0044] The cleaned quartz glass substrate is placed in a tube furnace, the pressure of the reaction chamber is pumped to below 10Pa, and then 500-1000sccm Ar and 500-1000sccm H are introduced 2 , the temperature is raised to 1000-1100°C; after the temperature is stabilize...

Embodiment 2

[0054] Embodiment 2: the use of graphene glass chip in graphene refractive index sensor

[0055] The graphene glass chip in the present invention is specially suitable for the refractive index sensor based on the polarized absorption characteristic of graphene under the condition of total reflection. Such as figure 2 As shown, the core of this type of refractive index sensor consists of an optical system, a graphene glass chip, and a microfluidic system. The prism, the graphene glass chip, and the microfluidic channel are assembled together in a sandwich structure. Specifically, the glass substrate side of the chip is bonded to the prism with a refractive index matching liquid, and the PDMS microfluidic channel is bonded to the graphite chip. On the graphene side, biomolecules bound to the graphene surface reside in microfluidic channels. Mount the assembled prism / graphene-glass chip / microfluidic channel on an e.g. image 3 In the detection optical system shown, a 532nm la...

Embodiment 3

[0056] Embodiment 3: the sensitivity analysis of graphene glass chip

[0057] On the graphene glass chip of Example 1, rabbit IgG molecules were coupled as target molecules, and after the chip was mounted on the refractive index sensor described in Example 2, a series of concentration gradient rabbit anti- IgG solution (0.1, 0.4, 1, 2.5, 10, 25, 100μg / ml), the combination of anti-IgG and IgG on the chip causes the refractive index of the graphene surface medium to change, so that the graphene is sensitive to S polarized light and P The absorption of polarized light changes, and the signal collected by the balance detector after polarization splitting also changes accordingly. From Figure 4 It can be seen that as the concentration of anti-IgG increases, the response value of the detector increases, and the detection sensitivity of the sensor to anti-IgG is 0.1 μg / ml. Figure 5 It is the result of detecting different concentrations of anti-IgG solutions using a commercial BIA...

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Abstract

The invention discloses a preparation method of a graphene glass chip. The method comprises the following steps: (1) cleaning a glass substrate; (2) growing graphene; (3) carrying out graphene surface treatment; (4) carrying out biomolecule modification, namely soaking the treated graphene glass in a buffer liquid containing biomolecules, and reacting at room temperature so as to couple the graphene surface with a layer of biomolecules; and (5) cleaning to obtain a graphene glass chip, wherein the obtained graphene glass chip is applied to a refractive index sensor based on the graphene polarization absorption effect in a total reflection condition. Compared with the graphene chip prepared by traditional transfer and redox method, the graphene glass chip has the advantages of easiness in preparation, strong binding force with the glass substrate, longer service life, accurate control on the graphene thickness, high repeatability, sensitive, accurate and stable detection result and the like.

Description

technical field [0001] The invention relates to the fields of refractive index sensing and biomolecular detection, in particular to a method for preparing a graphene glass chip applied to a refractive index sensor based on the graphene polarization absorption effect under total reflection conditions. Background technique [0002] Refractive index sensing and biomolecular detection have a wide range of application requirements in life sciences, medical testing, drug screening, environmental monitoring and other fields. SPR technology can track and detect the interaction between biomolecules in real time. In SPR equipment, the chip is the core component of signal detection, but due to the limited regeneration times of the chip, it becomes a consumable that needs to be replaced frequently, and the relatively high price of the SPR chip increases the cost of using the SPR instrument, pushing up It reduces the cost of testing and research and development, and brings a heavy burden...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/543G01N21/21G01N21/552
CPCG01N21/21G01N21/552G01N33/54366
Inventor 陈旭东刘忠范
Owner 北京石墨烯研究院有限公司
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