Eureka AIR delivers breakthrough ideas for toughest innovation challenges, trusted by R&D personnel around the world.

A detection method for biogenic amines in fish meat and its products

A technology for fish meat products and detection methods, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of low accuracy, poor reproducibility and stability, and achieve the effects of short time consumption, enhanced retention, and enhanced separation effect

Active Publication Date: 2020-07-24
GUANGDONG UNIV OF TECH
View PDF6 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these methods generally have problems such as low precision, poor reproducibility and stability

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A detection method for biogenic amines in fish meat and its products
  • A detection method for biogenic amines in fish meat and its products
  • A detection method for biogenic amines in fish meat and its products

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] 1. Preparation of biogenic amine standard solution

[0051] Accurately weigh the appropriate amount of 7 kinds of biogenic amine standard substances including tryptamine, putrescine, cadaverine, histamine, tyramine, spermidine and spermine, put them into seven 10mL volumetric flasks respectively, and then add 0.1mol / Dilute the L hydrochloric acid solution to the mark, mix well, and prepare a single biogenic amine standard with a concentration of 1000 mg / L. Then draw 1.00mL of a single biogenic amine standard, put it in the same volumetric flask, add 0.1mol / L hydrochloric acid to dilute to the mark, mix well, and prepare a biogenic amine standard solution with a final concentration of 10mg / L.

[0052] Take 0.5mL of biogenic amine standard, add 20μL of 1,7-diaminoheptane as internal standard, mix well so that the final concentration of internal standard is 100mg / L; then add 1.5mL of saturated sodium bicarbonate solution to adjust the pH value to Weakly alkaline, then ad...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the field of food analysis and safety and relates to a method for detecting biogenic amines in fish and fish products. The method combines precolumn derivatization with dansyl chloride, constructs chromatographic conditions and gradient elution processes for detecting biogenic amines in fish and fish products through reversed-phase high-performance liquid chromatography (RP-HPLC), realizes the optimal separation of various biological amines, is free of sample purification treatment in the whole experimental process, has a simple derivation process, reduces an active ingredient loss, has a measurement limit in a microgram / liter level, has short time consumption and realizes large-scale sample detection.

Description

technical field [0001] The invention belongs to the field of food analysis and safety, and in particular relates to a method for detecting biogenic amines in fish meat and its products. Background technique [0002] Biogenic amine is a general term for a class of nitrogen-containing basic organic compounds with low molecular weight. It contains a small amount of biogenic amine in the tissues of the human body, animals and plants. It is a normal active ingredient in biological organisms and plays an important physiological role in the body. effect. Putrescine, spermine, spermidine, and cadaverine play an important role in regulating the synthesis of nucleic acids and proteins and the stability of biofilms, and are essential parts of biologically active cells. At the same time, biogenic amines are also commonly found in a variety of foods and some fermented products. It is generally believed that most of the biogenic amines in food are produced by decarboxylation of amino aci...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/89
CPCG01N30/89
Inventor 陶志华胡琴霞陈泽熙段雪娟张宏梅
Owner GUANGDONG UNIV OF TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com