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A detection method for biogenic amines in fish meat and its products

A technology for fish meat products and detection methods, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of low accuracy, poor reproducibility and stability, and achieve the effects of short time consumption, enhanced retention, and enhanced separation effect

Active Publication Date: 2020-07-24
GUANGDONG UNIV OF TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, these methods generally have problems such as low precision, poor reproducibility and stability

Method used

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  • A detection method for biogenic amines in fish meat and its products
  • A detection method for biogenic amines in fish meat and its products
  • A detection method for biogenic amines in fish meat and its products

Examples

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Embodiment 1

[0050] 1. Preparation of biogenic amine standard solution

[0051] Accurately weigh the appropriate amount of 7 kinds of biogenic amine standard substances including tryptamine, putrescine, cadaverine, histamine, tyramine, spermidine and spermine, put them into seven 10mL volumetric flasks respectively, and then add 0.1mol / Dilute the L hydrochloric acid solution to the mark, mix well, and prepare a single biogenic amine standard with a concentration of 1000 mg / L. Then draw 1.00mL of a single biogenic amine standard, put it in the same volumetric flask, add 0.1mol / L hydrochloric acid to dilute to the mark, mix well, and prepare a biogenic amine standard solution with a final concentration of 10mg / L.

[0052] Take 0.5mL of biogenic amine standard, add 20μL of 1,7-diaminoheptane as internal standard, mix well so that the final concentration of internal standard is 100mg / L; then add 1.5mL of saturated sodium bicarbonate solution to adjust the pH value to Weakly alkaline, then ad...

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Abstract

The invention belongs to the field of food analysis and safety and relates to a method for detecting biogenic amines in fish and fish products. The method combines precolumn derivatization with dansyl chloride, constructs chromatographic conditions and gradient elution processes for detecting biogenic amines in fish and fish products through reversed-phase high-performance liquid chromatography (RP-HPLC), realizes the optimal separation of various biological amines, is free of sample purification treatment in the whole experimental process, has a simple derivation process, reduces an active ingredient loss, has a measurement limit in a microgram / liter level, has short time consumption and realizes large-scale sample detection.

Description

technical field [0001] The invention belongs to the field of food analysis and safety, and in particular relates to a method for detecting biogenic amines in fish meat and its products. Background technique [0002] Biogenic amine is a general term for a class of nitrogen-containing basic organic compounds with low molecular weight. It contains a small amount of biogenic amine in the tissues of the human body, animals and plants. It is a normal active ingredient in biological organisms and plays an important physiological role in the body. effect. Putrescine, spermine, spermidine, and cadaverine play an important role in regulating the synthesis of nucleic acids and proteins and the stability of biofilms, and are essential parts of biologically active cells. At the same time, biogenic amines are also commonly found in a variety of foods and some fermented products. It is generally believed that most of the biogenic amines in food are produced by decarboxylation of amino aci...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/89
CPCG01N30/89
Inventor 陶志华胡琴霞陈泽熙段雪娟张宏梅
Owner GUANGDONG UNIV OF TECH
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