Molecular gradient forward and reverse ultrafiltration separation and purification device and ultrafiltration method thereof

Abstract

The molecular gradient forward and reverse ultrafiltration separation and purification device disclosed in the present invention and its use method include an end sealer and at least two ultrafiltration units, and the ultrafiltration units include a tube body and an ultrafiltration membrane. The ultrafiltration membranes are arranged in the opposite direction, and the adjacent ultrafiltration units are detachably connected in the axial direction. The ultrafiltration membranes of the adjacent ultrafiltration units have different maximum molecular weights, and the ultrafiltration units at both ends are respectively connected to the end cappers. The invention can automatically separate and purify biomolecules in different intervals of molecular weight according to molecular gradient closed type, conveniently realize multiple times of forward and reverse purification, and facilitate graded collection of biomolecules in relevant intervals.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to the field of molecular ultrafiltration separation and purification. Background technique [0002] With the development of biotechnology, biological macromolecules such as small molecular proteins, polysaccharides or nucleic acids play an important role in biological effects, and most scholars are devoted to research in related fields. In order to accurately study the mechanism of action of a certain type of biomolecules, it must be separated and purified first, so as to achieve the purpose of targeted and precise research. To this end, it is necessary to invent a device that can separate biomolecules of different molecular weights. At present, there is a commercial product Amicon ultra-0.5 centrifugal filter devices (made in iceland) that can separate biomolecules larger or smaller than a certain molecular weight. Biomolecules are separated, that is, a filter membrane (such as 10,00...

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Problems solved by technology

[0004] (1) Biomolecules that require a molecular weight in a certain range require two operations, which is complicated, and the loss of biomolecules is large and the yield is low;

[0005] (2) Biomolecules with molecular weights in multiple intervals cannot be obtained at the same time;

[0006] (3) In order to minimize the incorporation of other molecular weight biomacromolecules, repeated operations are required, which is time-consuming and laborious. Multiple transfers of the solution will inevitably lead to a large loss of the target biomolecules. The waste of the research target biomacromolecules may cause the termination of the experiment or time delay

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