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Construction method of cordyceps militaris polysaccharide multi-dimensional fingerprint spectrum and standard fingerprint spectrum thereof

A standard fingerprint, Cordyceps militaris polysaccharide technology, applied in the field of fingerprints, can solve the problems of affecting the efficacy of polysaccharide active Cordyceps militaris materials, difficult to determine the fingerprint of unknown samples, affecting the quality of polysaccharides, etc., so as to protect consumers' rights and interests and promote quality. Improve and stabilize, reproducible effect

Active Publication Date: 2017-05-17
HEBEI UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Cordyceps militaris polysaccharides have been tested and analyzed, and they are mainly heteropolysaccharides composed of glucose, mannose, arabinose, galactose, rhamnose, and xylose. In addition, they also contain complexes such as proteins and nucleic acids. Cordyceps militaris has been reported The molecular weight of the purified polysaccharide ranges from thousands to hundreds of thousands of Daltons. Therefore, the polysaccharide of Cordyceps militaris is a complex macromolecular compound with a variety of special and quite complex chemical structures in most cases. Mainly manifested in: the type, molecular weight and distribution of monosaccharides that make up polysaccharides, the composition of polysaccharide complexes (proteins and nucleic acids), sugar chain structures, and advanced structures are all very complex, and it is difficult to identify general polysaccharides by single chemical analysis methods and instrumental analysis methods These factors will affect the quality of polysaccharides, thereby affecting the activity of polysaccharides and the efficacy of Cordyceps militaris medicinal materials. Therefore, how to control the quality of Cordyceps militaris polysaccharides with simple, fast and accurate methods is an urgent need a problem solved
[0004] The existing single fingerprint method has the disadvantage that it is difficult to overcome, that is, it is difficult to determine whether the unknown sample conforms to the fingerprint of the standard sample, and false positive results sometimes appear

Method used

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  • Construction method of cordyceps militaris polysaccharide multi-dimensional fingerprint spectrum and standard fingerprint spectrum thereof
  • Construction method of cordyceps militaris polysaccharide multi-dimensional fingerprint spectrum and standard fingerprint spectrum thereof
  • Construction method of cordyceps militaris polysaccharide multi-dimensional fingerprint spectrum and standard fingerprint spectrum thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0068] The method for constructing the multidimensional fingerprint of the polysaccharide of Cordyceps militaris comprises extracting the polysaccharide of Cordyceps militaris from Cordyceps militaris, and subjecting the extracted polysaccharide of Cordyceps militaris to UV absorption spectrum fingerprint analysis, Fourier transform infrared spectrum fingerprint analysis and high performance gel permeation chromatography fingerprint Analyze, obtain the UV spectrum standard fingerprint of Cordyceps militaris polysaccharide, the IR spectrum standard fingerprint and the multidimensional fingerprint construction method of HPGPC chromatographic standard fingerprint, comprise the steps:

[0069] Cordyceps militaris raw materials: 10 groups of Cordyceps militaris raw materials from different origins are from Guangdong, Jilin, Fujian, Hebei and other places.

[0070] (1) Preparation of polysaccharide from Cordyceps militaris

[0071] Take 50 g of dried Cordyceps militaris, crush them,...

Embodiment 2

[0109] Example 2, Multi-dimensional fingerprint analysis of a polysaccharide sample of Cordyceps militaris from a certain production area in Guangdong

[0110] 1. Preparation of polysaccharide from Cordyceps militaris from a place in Guangdong

[0111] Take 50 g of dried Cordyceps militaris from a place in Guangdong, crush them, add absolute ethanol at a ratio of 1 g to 10 mL of solid to liquid, extract at 80°C for 2 h, and repeat the extraction twice after filtration to remove the fat-soluble compounds contained therein. Ethanol was evaporated, and the residue of medicinal materials was added to distilled water according to the ratio of material to liquid: 1g: 20 mL, and extracted with a 260 W ultrasonic extractor at 80°C for 15 min, then extracted in a water bath at a constant temperature of 80°C for 2 h, and repeated extraction twice after filtration. The combined water extracts were concentrated under reduced pressure using a rotary evaporator to one-eighth of the volume o...

Embodiment 3

[0121] Example 3, Multidimensional Fingerprint Analysis of Cordyceps militaris Polysaccharide Sample from a Certain Production Area in Henan

[0122] 1. Preparation of polysaccharide from Cordyceps militaris from a place in Henan

[0123] Take 50 g of dried Cordyceps militaris from a place in Henan, crush it, add absolute ethanol at a solid-to-liquid ratio of 1 g: 10 mL, extract at 80 °C for 2 h, filter and repeat the extraction twice to remove the fat-soluble compounds contained in it. Ethanol was evaporated, and the residue of medicinal materials was added to distilled water according to the ratio of material to liquid: 1g: 20 mL, and extracted with a 260 W ultrasonic extractor at 80°C for 15 min, then extracted in a water bath at a constant temperature of 80°C for 2 h, and repeated extraction twice after filtration. The combined water extracts were concentrated under reduced pressure using a rotary evaporator to one-eighth of the volume of the water extracts. Add absolute ...

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Abstract

The invention discloses a construction method of a cordyceps militaris polysaccharide multi-dimensional fingerprint spectrum, which relates to the technical field of fingerprint spectrums of traditional Chinese medicine and functional food materials as well as products of the traditional Chinese medicine and the functional food materials. The construction method comprises the steps of extracting cordyceps militaris polysaccharide from cordyceps militaris and performing ultraviolet absorption spectrum fingerprint analysis, Fourier transform infrared spectroscopy fingerprint analysis and high performance gel permeation chromatography fingerprint analysis on the extracted cordyceps militaris polysaccharide so as to obtain a multi-dimensional fingerprint spectrum of the cordyceps militaris polysaccharide including an UV standard fingerprint spectrum, an IR standard fingerprint spectrum and HPGPC standard fingerprint spectrum. The method has the advantages of simplicity, stability and sensitivity in operation, high precision, good reproducibility and the like, the quality of the cordyceps militaris polysaccharide can be controlled from the integral feature of the chromatography, and a new scientific method is provided for the quality control and true and false identification of the cordyceps militaris.

Description

technical field [0001] The invention relates to the technical field of fingerprints of traditional Chinese medicine and functional food raw materials and their products, in particular to a method for controlling the quality of polysaccharides from Cordyceps militaris by using multi-dimensional fingerprints. Background technique [0002] Cordyceps militaris ( Cordyceps militaris) Also known as Cordyceps militaris, Cordyceps militaris, and Chrysalis pupae. Cordyceps militaris is a precious traditional Chinese medicinal material in my country. The people have long studied and recognized its medicinal value. In 1518, Li Shizhen recorded in "Compendium of Materia Medica" that cicadae can cure "children with convulsions, night crying, and heart palpitations". According to the textual research of Jing Yibing and others, the "Cicada Flower" at that time included Cicada Cordyceps ( C. sobolifera ) and Cordyceps militaris ( C. militaris ) and many other Cordyceps that parasitize ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N21/33G01N21/3563
CPCG01N21/33G01N21/3563G01N30/02G01N30/06G01N2021/3595
Inventor 景永帅张丹参吴兰芳
Owner HEBEI UNIVERSITY OF SCIENCE AND TECHNOLOGY
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