Compound feed additive capable of reducing swine manure odor and application thereof
A compound additive and odor technology, applied in applications, animal feed, animal feed, etc., can solve problems such as high odor, excessive nitrate content in groundwater, and feces pollution, and achieve reduced elimination, improved digestion and absorption, and reduced The Effect of Fecal Odor Pollution Status
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[0045] Example 1
[0046] (1) Bacillus subtilis (strain preservation number CGMCC 1.504) was cultured on LB liquid medium for 18 hours, and the colony density reached 1×10 11 CFU / mL, add appropriate amount of bran after drying and concentration (the weight ratio of bacterial solution and bran is 1:100), the concentration of Bacillus subtilis in the final product is 1×10 12 CFU / g. The prepared Bacillus subtilis has the same physical and chemical properties as described.
[0047] The LB liquid medium refers to weighing 10 g of tryptone, 5 g of yeast extract, and 5 g of sodium chloride, adding about 800 mL of deionized water, and fully stirring it evenly. Adjust the pH to 7.0 with sodium hydroxide, add deionized water to make the volume to 1 L, and sterilize at 121°C for 20 minutes.
[0048] (2) Preparation method of Yucca plant extract:
[0049] Yucca plants refer to the Yucca schidigera (Yucca schidigera) after harvest, cut into small pieces and mechanically crushed into fibers, soa...
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[0051] Example 2
[0052] Select 20 growing pigs weighing 40 kg and divide them into two treatments, each with 10 pigs. The pigs in the control group were fed with basic diet, and the pigs in the experimental group were fed with 5×10 5 CFU / g Bacillus subtilis, 80 g / ton Yucca plant extract. The test period was 20 days, and stool was collected once every 10 days, and the content of 3-methylindole was determined. The results of the determination are shown in Table 4. The weight ratio of Bacillus subtilis and Yucca extract is 1:7;
[0053] Table 4 Effects of different diet treatments on the content of 3-methylindole in pig manure, ng / g
[0054]
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[0055] Example 3.
[0056] Choose 20 fattening pigs weighing 60 kg and divide them into two treatments, each with 10 pigs. The pigs in the control group were fed with basic diet, and the pigs in the experimental group were fed with 5×10 8 CFU / g Bacillus subtilis, 60 g / ton Yucca plant extract. The test period was 20 days, and stool was collected once every 10 days, and the content of 3-methylindole was determined. The results of the determination are shown in Table 5. The weight ratio of Bacillus subtilis and Yucca extract is 1:7;
[0057] Table 5 Effects of different diet treatments on the content of 3-methylindole in pig manure, ng / g
[0058]
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