Method for using biochip for cell fluid experiment

Abstract

The invention provides a use method of a biological chip for a cell fluid experiment. The use method is characterized by comprising the following steps: 1, calculating a fluid flowing network of a cell culture area at an experimental fluid flowing speed according to shapes and specifications of selected chips; 2, classifying the selected chips into an experiment group and a contrast group, and culturing experimental cells under the same condition; 3, after the cells grow in an attachment manner, continuously injecting fluid which is required by the experiment and has a certain flowing speed; 4, detecting the differentiation situation of the cells under the acting force of the fluid through a high-speed high-definition camera; 5, at the end of the experiment, discharging waste liquid. The chip has the following beneficial effects that compared with a common chip, the chip is equipped with an extra culture detection unit under different mechanical experimental conditions of the fluid, and different shear forces are applied in a cell culture process, so as to realize instant and effective trace observation; real-time monitoring can be carried out within a period of time of detection, the fluid input rate can be controlled, and the detection reliability can be guaranteed; therefore, an experimental detection result can better reflect a real cell growth environment, and people can master the influence of the shear force on the cells.

Description

technical field [0001] The invention relates to a method for using a cell culture biochip dedicated for testing, and in terms of use, it is a method for using a cell culture biochip used in a fluid experiment in a cell culture experiment. Background technique [0002] There are many difficult problems in the traditional biochip technology, mainly in the fact that the traditional biochip uses isolated genes and protein molecules, which undergo a process of extraction, separation, purification, surface adhesion, molecular binding, etc. from the intracellular environment. After a series of treatments, the treated cells change, which cannot be observed and analyzed in time. Therefore, to change this situation, it is necessary to fundamentally change the design idea of ​​biochips and design a chip that can form real-time observation and comparison. In the study of cell differentiation and proliferation, cell mechanics experiments are often used to simulate in vivo fluids, and on...

AI Technical Summary

Problems solved by technology

In the study of cell differentiation and proliferation, cell mechanics experiments are often used to simulate in vivo fluids, and one of the effects of cell exploration on different shear forces is one of them, but there is currently a lack of cell culture biochips for shear force experiments , has achieved the ability to obtain the real-time response of cells to shear stress under simulated hydrodynamic conditions

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