A Cell Culture Biochip for Shear Force Experiment

Abstract

The invention relates to a cell culture biological chip for a shear force experiment. The cell culture biological chip is characterized in that four injection holes which are distributed in two rows are formed in one end of a rectangular chip; the injection holes and a V-shaped channel are respectively distributed along two long sides of the rectangular chip, and converge to two connecting channels, which are symmetrical at left and right, in a Y shape. The two connecting channels are respectively connected with a rectangular culture area and a tapered culture area. Compared with the existing design, the cell culture biological chip provided by the invention has the beneficial effects that compared with the common chip, the cell culture biological chip is provided with culture systems for different shear forces, so that real-time, effective and trace observation can be realized by applying the different shear forces in a stem cell culture process. After the cell culture biological chip is used, real-time monitoring can be carried out within a detection period, the input speed of nutrient substances can be controlled, and the reliability of detection is guaranteed, so that the results can better reflect a real stem cell growth environment, and the influence of the shear forces on stem cell differentiation is mastered.

Description

technical field [0001] The invention relates to a novel cell culture biochip, and in terms of use, it is a cell culture biochip used in cell culture experiments for shear force experiments. Background technique [0002] Biochip is a high-tech that has developed rapidly in the field of biomedical engineering in the past 20 years. Biochips are widely used in various fields such as life science research and practice, medical research and clinical practice, drug design, environmental protection, agriculture, and military affairs. Because of its high-throughput, miniaturization and automation characteristics, it conforms to the principles of fast, efficient, and low-cost industrial operation, and has very strong application prospects in the fields of disease diagnosis, drug screening, and cell culture. These will undoubtedly produce huge social and economic benefits. However, there are many difficult problems in the traditional biochip technology, mainly in that the traditional...

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Problems solved by technology

[0003] In the study of cell differentiation and proliferation, cell mechanics experiments are often used to simulate in vivo fluids, and one of the effects of cell exploration on different shear forces is one of them, but there is currently a lack of cell culture for shear force experiments Biochips lack the real-time response of cells to shear stress under simulated hydrodynamic conditions

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