A method of using a biochip for shear force experiments

Abstract

The invention discloses a use method of a biological chip used for a shear force experiment. An operation step comprises the following steps of 1, choosing chips according to chip specification selected by a cell shear force experiment; 2, inputting all part dimensions of the selected chips into a computer to prepare a fluid flowing network; 3, dividing the selected chips into an experiment group and a comparison group to perform experiment cell culture; and 4, performing analysis on the collected cell experimental data according to cell positioning and fluid network positioning, and researching on influence on the cells from the fluid shear force. The chip disclosed by the invention has the following beneficial effects: compared with a common chip, a culture system with different shear forces is set for the biological chip; therefore, different shear forces are applied in a stem cell culturing process, so that in-time, effective and micro observation can be achieved; and in addition, real-time detection in a detection time frame can be carried out, the input rate of nutrient substances can be controlled, and detection reliability is ensured, so that the result can reflect the stem cell growth environment more actually, and the influence on stem cell differentiation by the shear force can be mastered.

Description

technical field [0001] The invention relates to a method for using a novel cell culture biochip, which is a method for using a cell culture biochip used in a cell culture experiment for shear force experiments. [0002] technical background [0003] Biochip is a high-tech that has developed rapidly in the field of biomedical engineering in the past 20 years. Biochips are widely used in life science research and practice, medical research and clinical, drug design, environmental protection, agriculture, military and other fields. Because of its high-throughput, miniaturization and automation characteristics, it conforms to the principles of rapid, efficient, and low-cost industrial operation, and has very strong application prospects in the fields of disease diagnosis, drug screening, and cell culture. These will undoubtedly produce huge social and economic benefits. However, there are many problems that are difficult to solve in traditional biochip technology, mainly in tha...

AI Technical Summary

Problems solved by technology

[0004] In the study of cell differentiation and proliferation, cell mechanics experiments are often used to simulate in vivo fluids, and one of the effects of cell exploration on different shear forces is one of them, but there is currently a lack of cell culture for shear force experiments Biochips have achieved the ability to obtain real-time responses of cells to shear stress under simulated hydrodynamic conditions

Images