Wide-view-field multi-scale high-resolution microimaging system and method

Abstract

The invention discloses a wide-view-field multi-scale high-resolution microimaging system, and the system comprises a light source, a sample, a microimaging object lens, a collection system, and a work station. The microimaging object lens is used for carrying out the imaging after a light beam emitted by the light source irradiates the sample, and obtaining a wide-view-field curved image plane. The collection system comprises a collection array for collecting the curved image plane according to the view field, and a camera array for secondary imaging. The work station is used for carrying out the analysis of an imaging overlapping view field obtained through collection and obtaining a final multi-scale wide-view-field high-resolution image through view field splicing. The invention also discloses a wide-view-field multi-scale high-resolution microimaging method. Through the design of the object lens and the improvement of an imaging method, the system achieves the multi-scale high-resolution observation of cells, tissues and organs while guaranteeing the wide view field.

Description

technical field [0001] The invention belongs to the field of optical microscopy technology, and in particular relates to the design and method of a wide-field multi-scale and high-resolution microscopic imaging system. Background technique [0002] With the development of science and technology, people are constantly pursuing larger and larger observation ranges and higher resolution capabilities in the research of neuropathology, genetics, and neuroscience research. [0003] In the design of conventional imaging instruments, the analysis is mainly focused on the case where the object plane and the image plane are planes. Usually, the sequential independent mode from image point to object point is adopted, and only the mapping design of image point to object point is considered. In the calculation of light field design, the imaging relationship between the optical system, the photoelectric system and the signal processing system is integrated, and the system performance is j...

AI Technical Summary

Problems solved by technology

The optical imaging instrument based on the principle of computational optics not only needs to be able to accurately observe the biological characteristics of a single cell itself, but also has the ability to evaluate pathophysiological processes and mutual influences in the body's complete microenvironmental state, which is expected to reveal physiological and the laws of important life phenomena under pathological conditions, which put forward strict requirements and challenges for the development of observation optical instruments

Images