A kind of cell metabolism quenching system and its cell metabolism quenching method

Abstract

The invention relates to a cell metabolism quenching system and a cell metabolism quenching method thereof. The cell metabolism quenching system comprises a cell supply device, a liquid and liquid mixing device, a cell collecting device and an electromagnetic feedback control device. The cell metabolism quenching system has the advantages that under the combined action of the liquid and liquid mixing device, the cell collecting device and the electromagnetic feedback control device, cell culture liquid is fully mixed with a low-temperature cell quenching solution, cells can be instantaneously cooled to low temperature, the activity of enzyme is effectively inhibited, and the metabolism quenching is thorough; by using the electromagnetic feedback control device and a vacuum pump, the concentration of a quenching solvent at an end point is controllable, the volume ratio of the cell culture liquid to the low-temperature cell quenching solution is accurately controlled, data of intracellular metabolite is true and reliable, the synergistic action of all the devices is realized, and the metabolism quenching is timely and thoroughly performed. on the cells The cell metabolism quenching method by using the cell metabolism quenching system has the advantages that the metabolism quenching is simple, the implementing is easy, the accuracy rate is high, and technical support is provided for the study on the intracellular metabolite of microorganism, animal and plant cells.

Description

technical field [0001] The invention relates to a biological cell metabolism quenching device and a cell metabolism quenching method thereof, and relates to the technical fields of microorganisms, animals, plants, medicine and chemical industry. Background technique [0002] The field of biotechnology has developed by leaps and bounds in the past few decades, opening up a new era, and the analysis technology of omics research with genomics, transcriptomics, proteomics and metabolomics as important components is playing an important role . Like the transcriptome and proteome, metabolomics also depends on the biological background, and the concentration of each metabolite depends on the physiological and biochemical state of the cell, tissue or body. However, unlike mRNA and proteins, establishing the link between genes and metabolites is extremely difficult or even impossible at the state of the art. A gene can be transcribed to form multiple mRNAs, a piece of mRNA can be t...

AI Technical Summary

Problems solved by technology

In the research of metabolomics, due to the incomplete quenching of cell metabolism, the activity of cell enzymes cannot be inhibited in time, which leads to the conversion or decomposition of target metabolites, resulting in large deviations in experimental results.

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