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Magnetic separation device and separation method thereof

A magnetic separation and magnetic core technology, applied in biochemical equipment and methods, methods of stress stimulating microbial growth, treatment of microorganisms with electricity/wave energy, etc., can solve the problem of magnetic field intensity attenuation, magnetic field inhomogeneity, target cell loss, etc. problem, to achieve the effect of reducing loss, good adsorption effect, and increasing the strength of the magnetic field

Active Publication Date: 2017-05-31
上海顿慧医疗科技发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

A commonly used method in CTC enrichment technology is the technology with immunomagnetic enrichment as the core technology. The current commercial CTC enrichment and detection technology, such as CellSearch, uses EpCAM magnetic beads to label CTC and perform magnetic screening, but its adsorption magnetic The magnet of the beads is set outside the sample test tube, so the magnetic field in the test tube is not uniform, and the magnetic field strength attenuates significantly with the increase of the distance of the magnetic field, resulting in almost zero magnetic force at the end far away from the magnet, and the target cells at this end are easily sucked and killed. loss

Method used

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  • Magnetic separation device and separation method thereof
  • Magnetic separation device and separation method thereof
  • Magnetic separation device and separation method thereof

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Embodiment 1

[0050] Figure 1-4 Shown is a schematic structural view of a preferred embodiment of the magnetic separation device of the present invention, the magnetic separation device comprises a cylindrical insertion rod 1, a magnetic core 2 is arranged at the bottom end of the insertion rod 1, and the inside of the magnetic core is wrapped with Magnet, this magnet is made of permanent magnet 21 and the soft magnetic material 22 that wraps it outside ( image 3 ), the side surface of the permanent magnet 21 that only exposes the part corresponds to the magnetic field transmission window on the insertion rod 1 ( figure 2 The rectangular area in the middle) is used to capture the target magnetic particles. The transmission window can be hollowed out or made of transparent magnetic material, such as glass; Made of transparent or opaque material.

[0051] In addition, this magnetic separation device also includes an outer sheath 3 ( Figure 4 ), the outer sheath 3 is enclosed within the...

Embodiment 2

[0054] Figure 5 Shown is a schematic structural view of another preferred embodiment of the magnetic separation device of the present invention, the magnetic separation device includes a cylindrical insertion rod 1, the bottom end of the insertion rod 1 has a magnetic core 2, and four magnetic cores are arranged in the magnetic core. A small cube or cuboid magnet, each of which is composed of permanent magnets wrapped by soft magnetic materials on five outer surfaces, the unwrapped side corresponds to the magnetic field transmission window of the magnetic core and the capture observation window 4 on the outer sheath 3, And the magnetic poles of two adjacent permanent magnets are the same (such as Figure 6a in N-S-N-S-N-S-N-S) or vice versa (eg Figure 6b N-S-S-N-N-S-S-N in the middle); In addition, the insertion rod 1 has a protrusion 5 near the upper end, which can be used as a locking part, so that the device can be hung on the support frame conveniently. The arrangement...

Embodiment 3

[0056] Figure 7 Shown is a schematic structural view of another preferred embodiment of the magnetic separation device of the present invention, wherein the bottom end of the insertion rod 1 is connected with a magnetic core 2, and the upper end 11 of the insertion rod 1 is thick cylindrical, which is easy to hold. The middle section 12 of 1 is thin cylindrical, when this device is inserted in the liquid sample, this kind of design can reduce the overflow of liquid.

[0057] Figure 8 Shown is the schematic diagram of the magnetic core 2 when the outer sheath 3 is sleeved in this embodiment, Figure 9 and Figure 10 are respectively the schematic diagrams of the magnetic core 2 and the outer sheath 3 separated, from Figure 9 It can be seen in the figure that the magnetic core 2 has a housing groove 23, a magnet is installed in the housing groove, and a magnetic field transmission window is arranged on the housing groove 23. The magnet is composed of a permanent magnet 21 ...

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Abstract

The invention discloses a magnetic separation device. The device comprises a magnetic core and an inserting rod, wherein the magnetic core is arranged at one end of the inserting rod; and a capture plane of the side surface of the magnetic core can send out a magnetic field for adsorbing target magnetic particles in a liquid sample outwards. Through the design of the magnetic core, the target magnetic particles can be intensively adsorbed in a relatively small plane area on the surface of the magnetic core; and the size and the shape of the area can be designed according to the requirements, so that the effect of concentrating target materials is generated, and the magnetic separation device is especially suitable for capture and collection of rare materials in the sample.

Description

technical field [0001] The invention relates to a magnetic sorting device and a sorting method thereof, in particular to a magnetic sorting device and a sorting method suitable for capturing rare cells. Background technique [0002] Immunomagnetic enrichment is the use of cell surface antigens to bind to specific monoclonal antibodies connected to magnetic beads. Under the action of an external magnetic field, cells connected to magnetic beads through antibodies are attracted and retained in the magnetic field, while other cells are trapped in the magnetic field due to Non-magnetic and cannot stay in a magnetic field, allowing cells to separate. Among them, the more representative method is the magnetic activated cell sorting system (MACS), which uses immunomagnetic beads to mark cell membrane or intracellular cell antigens, and then captures the target labeled by the immunomagnetic beads through an external magnetic field. cell. [0003] Circulating tumor cells (CTCs) ref...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/42C12N13/00C12N5/00
CPCC12M35/02C12N5/00C12N13/00
Inventor 樊嘉周俭杨欣荣孙云帆
Owner 上海顿慧医疗科技发展有限公司