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A washing-free magnetic separation device and its separation method

A magnetic separation and magnetic core technology, applied in the field of wash-free magnetic separation device for rare cell capture, to achieve the effects of reduced loss, good adsorption effect, and high capture efficiency

Active Publication Date: 2019-08-02
上海顿慧医疗科技发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Another method of adsorbing magnetic beads is to use a magnetic rod inserted into the test tube. The bottom surface of the magnetic rod is magnetic. Although this method can greatly improve the efficiency of magnetic field adsorption, the disadvantage of this method is that the cells must be eluted after adsorption. proceed to the next step

Method used

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  • A washing-free magnetic separation device and its separation method
  • A washing-free magnetic separation device and its separation method
  • A washing-free magnetic separation device and its separation method

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Experimental program
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Embodiment 1

[0051] Figure 1-4 Shown is a schematic structural view of a preferred embodiment of the washing-free magnetic separation device of the present invention, the magnetic separation device comprises a cylindrical insertion rod 1, and a magnetic core 2 is arranged at the bottom end of the insertion rod 1, the magnetic core The inside is wrapped with a magnet, which is made of a permanent magnet 21 and a soft magnetic material 22 wrapped outside it ( image 3 ), the side surface of the permanent magnet 21 that only exposes the part corresponds to the magnetic field transmission window on the insertion rod 1 ( figure 2 The rectangular area in the middle) is used to capture the target magnetic particles. The transmission window can be hollowed out or made of transparent magnetic material, such as glass; Made of transparent or opaque material.

[0052] In addition, this no-elution magnetic separation device also includes an outer sheath 3 ( Figure 4 ), the outer sheath 3 is enclo...

Embodiment 2

[0055] Figure 6 Shown is a schematic structural view of another preferred embodiment of the washing-free magnetic separation device of the present invention, the washing-free magnetic separation device includes a cylindrical insertion rod 1, the bottom end of the insertion rod 1 has a magnetic core 2, the There are four cube or cuboid small magnets inside the magnetic core, and each magnet is composed of permanent magnets with five outer surfaces wrapped by soft magnetic materials, and the unwrapped side corresponds to the magnetic field transmission window of the magnetic core and the outer sheath 3 The capture observation window 4, and the magnetic poles of the adjacent two permanent magnets are consistent (such as Figure 7a in N-S-N-S-N-S-N-S) or vice versa (eg Figure 7b N-S-S-N-N-S-S-N in the middle); In addition, the insertion rod 1 has a protrusion 5 near the upper end, which can be used as a locking part, so that the device can be hung on the support frame convenien...

Embodiment 3

[0057] Figure 8 Shown is a schematic structural view of another preferred embodiment of the magnetic separation device of the present invention, wherein the bottom end of the insertion rod 1 is connected with a magnetic core 2, and the upper end 11 of the insertion rod 1 is thick cylindrical, which is easy to hold. The middle section 12 of 1 is thin cylindrical, when this device is inserted in the liquid sample, this kind of design can reduce the overflow of liquid.

[0058] Figure 9 Shown is the schematic diagram of the magnetic core 2 when the outer sheath 3 is sleeved in this embodiment, Figure 10 and Figure 11 are respectively the schematic diagrams of the magnetic core 2 and the outer sheath 3 separated, from Figure 10 It can be seen in the figure that the magnetic core 2 has a housing groove 23, a magnet is installed in the housing groove, and a magnetic field transmission window is arranged on the housing groove 23. The magnet is composed of a permanent magnet 2...

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Abstract

The invention discloses an elution-free magnetic separation device comprises a magnetic core and a capture view window arranged outside the magnetic core, wherein the capture view window is prepared from a magnetic transmitting material; the capture view window is the unique transmitting area of the magnetic field emitted by the magnetic core; the magnetic field emitted by the magnetic core is emitted out through the capture view window, and used for adsorbing target magnetic particles in the liquid sample; and the capture view window is detachable. When the separation device is used for separating the magnetic target particles in the liquid sample, the magnetic field emitted by the magnetic core adsorbs the target particles to the surface of the capture view window; and the captured target substance can be directly used for the next analysis and research only by taking down the capture view window without elution, thereby saving the operation steps, and reducing the loss of the target substance. The operating method is convenient and quick, and is very suitable for separation of rare samples in various biological samples.

Description

technical field [0001] The invention relates to a washing-free magnetic separation device and a sorting method thereof, in particular to a washing-free magnetic separation device suitable for capturing rare cells and a sorting method thereof. Background technique [0002] Immunomagnetic enrichment is the use of cell surface antigens to bind to specific monoclonal antibodies connected to magnetic beads. Under the action of an external magnetic field, cells connected to magnetic beads through antibodies are attracted and retained in the magnetic field, while other cells are trapped in the magnetic field due to Non-magnetic and cannot stay in a magnetic field, allowing cells to separate. Among them, the more representative method is the magnetic activated cell sorting system (MACS), which uses immunomagnetic beads to mark cell membrane or intracellular cell antigens, and then captures the target labeled by the immunomagnetic beads through an external magnetic field. cell. [...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/42C12N13/00C12N5/00
CPCC12M35/02C12N5/00C12N13/00
Inventor 樊嘉周俭杨欣荣孙云帆
Owner 上海顿慧医疗科技发展有限公司