A method for compound microbial fermentation to improve the quality of tobacco flakes

A technology of composite microorganisms and composite microorganisms, which is applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of single aroma, insufficient aroma, and no natural rich aroma, etc., to improve the quality of thin slices, aroma Naturally enriched, irritation-reducing effect

Active Publication Date: 2020-05-08
HUBEI CHINA TOBACCO IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] However, the tobacco leaves fermented by a certain type or a certain type of microorganisms have a slightly monotonous aroma, and the aroma is not rich enough. There is no natural rich aroma brought by different fermentations or reactions during the natural fermentation process with seasonal temperature changes.
[0011] At present, there are many biological fermentation methods in the method of aging tobacco leaves, but for the method of improving the quality of tobacco sheets, there are now many methods of applying various extracts to the surface of the sheet or coating the surface of the sheet to improve the quality, and No biological fermentation method has been used

Method used

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  • A method for compound microbial fermentation to improve the quality of tobacco flakes
  • A method for compound microbial fermentation to improve the quality of tobacco flakes
  • A method for compound microbial fermentation to improve the quality of tobacco flakes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Place the lactic acid bacteria and golden flower strains at a temperature of 20°C for 4 hours, then inoculate the lactic acid bacteria and golden flower strains on the activated medium according to the inoculation amount of 1% of the weight of the medium, and cultivate them at 20°C. For 48 hours, the bacterial seeds were prepared; the lactic acid bacteria and the golden flower bacteria were mixed according to a certain ratio, so that the number of live bacteria of the lactic acid bacteria was 5×10 7 cFu / ml, the number of live bacteria of Jinhua bacteria is 4×10 7 cFu / ml.

[0024] Take 1Kg of tobacco flakes and put them into the warehouse of aged tobacco leaves, evenly disperse the tobacco flakes, and evenly spray the compound microbial bacterial liquid of lactic acid bacteria and golden flower bacteria on the surface of the tobacco flakes. The amount of bacterial liquid applied is 1% of the weight of the tobacco flakes % fermented for 5 days, and the temperature was co...

Embodiment 2

[0035] Place the lactic acid bacteria and golden flower strains at a temperature of 30°C for 2 hours, then inoculate the lactic acid bacteria and golden flower strains on the activated medium according to the inoculation amount of 3% of the weight of the medium, and cultivate them at 30°C. After 24 hours, the bacteria seeds were prepared; the lactic acid bacteria and the golden flower bacteria were mixed according to a certain ratio, so that the number of live bacteria of the lactic acid bacteria was 1×10 7 cFu / ml, the number of live bacteria of Jinhua bacteria is 1×10 7 cFu / ml.

[0036] Take 1Kg of tobacco flakes and put them into the warehouse of aged tobacco leaves, evenly disperse the tobacco flakes, and evenly spray the compound microbial bacterial liquid of lactic acid bacteria and golden flower bacteria on the surface of the tobacco flakes. % Fermented for 9 days, the temperature was controlled at 32°C.

[0037] Finally, the tobacco sheets are naturally stored at room...

Embodiment 3

[0046]Place the lactic acid bacteria and golden flower strains at a temperature of 35°C for 3 hours, then inoculate the lactic acid bacteria and golden flower strains on the activated medium according to the inoculation amount of 5% of the weight of the medium, and cultivate them at 25°C. For 48 hours, the bacterial seeds were prepared; the lactic acid bacteria and the golden flower bacteria were mixed according to a certain ratio, so that the number of live bacteria of the lactic acid bacteria was 3×10 8 cFu / ml, the number of live bacteria of Jinhua bacteria is 5×10 8 cFu / ml.

[0047] Take 1Kg of tobacco flakes and put them into the warehouse of aged tobacco leaves, spread the tobacco flakes evenly, and evenly spray the composite microbial bacterial liquid of lactic acid bacteria and golden flower bacteria on the surface of the tobacco flakes, the amount of bacterial liquid applied is 5% of the weight of the tobacco flakes %, fermented for 6 days, and the temperature was con...

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Abstract

The invention provides a method for improving the quality of tobacco flakes through compound microbial fermentation. The compound microbial liquid of lactic acid bacteria and golden flower fungus is evenly sprayed on the surface of tobacco thin slices, the amount of bacterial liquid applied is 1-5%, and the fermentation is carried out for 5-10 days. The temperature is controlled at 25-45°C. The tobacco flakes are then naturally stored at room temperature at 10-30°C for 1-4 weeks. After fermented by the compound microorganisms of lactic acid bacteria and golden flower bacteria in the present invention, the components in the tobacco sheet that are not conducive to cigarette smoking quality such as cellulose, protein, and pectin are effectively degraded, and the quality of the sheet is significantly improved, and the irritation is reduced. The sour aroma of tobacco, rich and natural aroma.

Description

technical field [0001] The invention relates to a biological fermentation technology, in particular to a method for improving the quality of tobacco flakes through compound microbial fermentation. Background technique [0002] Tobacco flakes are made from raw materials such as tobacco powder, tobacco leaf fragments, tobacco stems or low-grade tobacco leaves produced during the production and processing of ordinary tobacco leaves, and are reprocessed by papermaking methods, also known as reconstituted tobacco leaves or homogeneous tobacco leaves. Tobacco flakes are therefore different from normal tobacco leaves in that they do not grow naturally, and their smoking quality is generally poor. [0003] There are three kinds of tobacco leaf fermentation processes that are mostly used at present: (1) natural fermentation, (2) artificial fermentation, and (3) stacking fermentation. [0004] Natural fermentation (also known as natural alcoholization or aging) is the mildest ferment...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P1/02C12P1/04A24B15/20C12R1/25C12R1/645
CPCA24B15/20C12P1/02C12P1/04C12N1/145C12N1/205C12R2001/25C12R2001/645
Inventor 王珊庞登红喻世涛尹团章张磊苗丽坤
Owner HUBEI CHINA TOBACCO IND
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