Method for extracting high-UFA content fish oil through biological enzymolysis

Abstract

The invention discloses a method for extracting high-UFA content fish oil through biological enzymolysis. The fish oil is extracted from a rainbow trout bone containing a relatively high content of fish oil by adopting a compound protease technology and the extraction rate is high. The method comprises the steps of (1) cleaning and purifying the fish bone, draining, dicing and refrigerating; (2) carrying out high-temperature stewing; (3) carrying out cooling and homogenizing; (4) carrying out enzymolysis, adding 500-3,000U / g of compound protease at 50-70 DEG C, continuously stirring, and adjusting the pH value to be 5-9 once every 5-30min, wherein the enzymolysis time is 2-4h; (5) centrifuging an enzymatic hydrolysate while it is hot; and (6) centrifuging and then collecting the top layer of fish oil. Enzymolysis is carried out by adopting the compound protease, the oil yield can reach 60-90%, the extracted rainbow trout oil is jacinth, the oil is clear and transparent, the high-UFA content fish oil is pure in fragrance, free of peculiar smell, low in moisture and low in content of volatile substances.

Description

technical field [0001] The invention relates to a method for biological enzymatic hydrolysis of fish oil with increased UFA content. Background technique [0002] Fish oil is the general term for all oils in the fish body. It comes from the body of large marine fish. Its main components are Omega-3 fatty acids (including DHA and EPA), which are polyunsaturated fatty acids that can reduce inflammation. Lower blood fat, prevent cardiovascular disease. There are many ways to produce fish oil, such as heating and cooking, washing with light lye, solvent extraction, etc. Some of these methods extract low fish oil content, some have complex equipment, and improper handling may even cause environmental pollution. [0003] At present, the commonly used oil extraction methods are: pressing method, cooking method, organic solvent extraction method, light lye cleaning method, biological enzyme method, supercritical fluid extraction method. [0004] The heating cooking method is to de...

AI Technical Summary

Problems solved by technology

However, supercritical fluid extraction is mainly used to extract components containing high free fatty acids, and it is very difficult to achieve large-scale and industrialization. On the one hand, the process design is not easy to grasp, and multiple experiments are required to obtain necessary data; on the other hand, On the one hand, the cost of equipment purchase is huge, while the yield of equipment is relatively low, and the energy consumption is also large