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Reagents, methods and applications for detection of highly pathogenic h7 avian influenza virus

An avian influenza virus, highly pathogenic technology, applied in the direction of microbial-based methods, biochemical equipment and methods, microbial measurement/testing, etc., can solve the problem of H7 avian influenza virus highly pathogenic strains and low pathogenicity To achieve the effect of protecting health and life safety and reducing economic losses

Active Publication Date: 2020-04-14
SOUTH CHINA AGRI UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing detection and identification methods can usually only identify H7 subtype and N subtype, and cannot distinguish highly pathogenic strains and low pathogenic strains of H7 avian influenza virus

Method used

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  • Reagents, methods and applications for detection of highly pathogenic h7 avian influenza virus
  • Reagents, methods and applications for detection of highly pathogenic h7 avian influenza virus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] In this case, a specific detection probe was designed based on the specific recognition sequence of the highly pathogenic H7 avian influenza virus sequence shown in Seq ID No.1, and real-time fluorescent RT-PCR detection was performed on the highly pathogenic H7 avian influenza virus. as follows.

[0035] (1) Extraction of sample RNA

[0036] In this example, H1 subtype avian influenza virus, H3 subtype avian influenza virus, H4 subtype avian influenza virus, H5 subtype avian influenza virus, H6 subtype avian influenza virus, H8 subtype avian influenza virus, H9 subtype were extracted respectively. Avian influenza virus, H11 subtype avian influenza virus, highly pathogenic H7 subtype avian influenza virus, low pathogenic H7 subtype avian influenza virus, Newcastle disease virus, chicken infectious bronchitis virus, chicken infectious laryngotracheitis RNA from viruses and reoviruses were tested. All virus samples were provided by South China Agricultural University. ...

Embodiment 2

[0057] In this example, on the basis of Example 1, a specific amplification primer pair is designed according to the highly pathogenic H7 avian influenza virus specific recognition sequence of the sequence shown in Seq ID No.1, and the upstream primer is the sequence shown in Seq ID No.5 , the downstream primer is the sequence shown in SeqID No.6, and an H7 avian influenza virus probe is designed in addition, the sequence shown in Seq ID No.7;

[0058] Seq ID No.5: 5'-CCAAAGAGAAAACGGACTGCG-3'

[0059] Seq ID No. 6: 5'-CCATACCAACCATCAATTAGGCC-3'.

[0060] Seq ID No.7: 5'(FAM)-GGCCTATTTGGTGCTATAGCGGGTT-(BHQ1)3'

[0061] It should be noted that the upstream primer of the sequence shown in Seq ID No.5 is designed for the specific recognition sequence of highly pathogenic H7 avian influenza virus, that is, the upstream primer of the sequence shown in Seq ID No.5 contains Seq The sequence shown in ID No.1 can ensure the specificity of amplification; as for the downstream primers a...

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Abstract

The invention discloses a reagent and method for highly pathogenic H7 avian influenza virus detection and application of the reagent. The reagent for highly pathogenic H7 avian influenza virus detection in the present application comprises a primer pair and / or a probe. The primer pair and / or the probe is designed for a specific recognition sequence of highly pathogenic H7 avian influenza virus. The specific recognition sequence is a sequence shown in Seq ID No. 1, or a reverse complementary sequence of the sequence shown in Seq ID No. 1, or a synonymous mutation sequence of the sequence shown in Seq ID No. 1 or the reverse complementary sequence of the sequence shown in Seq ID No. 1, or a sequence substituted with 1-4 bases. The reagent in the present application can effectively distinguish the highly pathogenic H7 avian influenza virus from low pathogenic H7 avian influenza virus, thereby providing technical support and guidance for timely taking effective prevention and control measures, and reducing economic losses in livestock and poultry industry due to a failure in distinguishing between high pathogenicity and low pathogenicity of the H7 avian influenza virus.

Description

technical field [0001] The application relates to the field of detection of avian influenza virus, in particular to a reagent, method and application for detection of highly pathogenic H7 avian influenza virus. Background technique [0002] Avian influenza is an acute infectious disease caused by a subtype of influenza A virus, that is, avian influenza virus. It can also infect humans and is classified as a Class A infectious disease by OIE. The H7 subtype avian influenza virus is a new type of avian influenza virus that can infect both poultry and humans; the more famous one is the H7N9 strain. According to the statistics of the World Health Organization (WHO), since the first report of human infection with H7N9 subtype avian influenza in China in March 2013, as of January 18, 2017, a total of 918 laboratory-confirmed cases of human infection with H7N9 subtype avian influenza have been reported. , the mortality rate exceeds 10%. Since the beginning of winter in 2016, the ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/70C12Q1/686C12N15/11C12R1/93
CPCC12Q1/701
Inventor 廖明曹琛福贾伟新林彦星谢淑敏亓文宝花群义卢体康
Owner SOUTH CHINA AGRI UNIV
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