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Euglena gracilis culturing method

A technology of Euglena teniculata and its cultivation method, which is applied in the field of cultivating Euglena tenabilis, can solve the problems of long lag period, waste of glucose concentration, and lower culture yield, and achieve high yield utilization and lower production cost

Inactive Publication Date: 2017-06-20
HENAN NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This technology helps create plants faster by producing more nutrients from their roots than traditional methods like watering them down or adding fertilizers during winter months.

Problems solved by technology

This patented describes different types of plants called Euglenia gigas (Gigola). These vegetables have many beneficial uses including food or medicine making it easier to grow and produce compared to other crops like wheat grain crop. They also provide an excellent resource because they contain various compounds from their roots, providing important benefits beyond what was previously appreciated. Additionally, these plants require specific chemical substances when growing outside environments where there may lack natural resources like sugar.

Method used

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  • Euglena gracilis culturing method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Aerotrophic cultures:

[0023] In a 1000mL culture bottle, aerated autotrophic culture was carried out under the following conditions: the inoculation density of Euglena gracilis was 0.33g / L; the composition of the heterotrophic medium included: NaNO 3 1.5g / L, K 2 HPO 3 0.3g / L, KH 2 PO 3 0.2g / L, MgSO 4 ·7H 2 O 0.1g / L, TE trace element solution 1mL / L, the rest is water, and the formula of TE solution is H 3 BO 3 2.81g / L, MnCl 2 4H 2 O1.82g / L, ZnSO 4 ·7H 2 O 0.23g / L, Na 2 MoO 4 2H 2 O 0.34g / L, CuSO 4 ·5H 2 O 0.05g / L and Co(NO 3 ) 2 ·6H 2 O 0.06g / L; culture temperature 28°C, air flow 1.0L / min, add 0.05% silicone defoamer. The results are shown in Figure 1.

[0024] Aerated Heterotrophic Culture with Additional Carbon Source Glucose

[0025] In a 1000mL culture bottle, aerated heterotrophic culture was carried out with the addition of carbon source glucose. The culture conditions were: the inoculation density of Euglena gracilis was 0.33g / L; 3 1.5g / L, ...

Embodiment 2

[0028] Aerotrophic cultures:

[0029] In a 1000mL culture bottle, aerated autotrophic culture was carried out under the following conditions: the inoculation density of Euglena gracilis was 0.33g / L; the composition of the heterotrophic medium included: NaNO 3 1.5g / L, K 2 HPO 3 0.3g / L, KH 2 PO 3 0.2g / L, MgSO 4 ·7H 2 O 0.1g / L, TE trace element solution 1mL / L, the rest is water, and the formula of TE solution is H 3 BO 3 2.81g / L, MnCl 2 4H 2 O1.82g / L, ZnSO 4 ·7H 2 O 0.23g / L, Na 2 MoO 4 2H 2 O 0.34g / L, CuSO 4 ·5H 2 O 0.05g / L and Co(NO 3 ) 2 ·6H 2 O 0.06g / L; culture temperature 25°C, air volume 1.0L / min, add 0.05% silicone defoamer. The results are shown in Figure 1.

[0030] Aerated Heterotrophic Culture with Additional Carbon Source Glucose

[0031] In a 1000mL culture bottle, aerated heterotrophic culture was carried out with the addition of carbon source glucose. The culture conditions were: the inoculation density of Euglena gracilis was 0.33g / L; 3 1.5g / L...

Embodiment 3

[0034] Heterotrophic culture with additional carbon source glucose aeration, feeding and rehydration culture in the early stage of the exponential phase.

[0035] In a 1000mL culture bottle, aerated heterotrophic culture was carried out with the addition of carbon source glucose. The culture conditions were: the inoculation density of Euglena gracilis was 0.33g / L; the composition of the heterotrophic medium included: glucose 20g / L, yeast powder 5g / L , K 2 HPO 3 0.3g / L, KH 2 PO 3 0.2g / L, MgSO 4 7H2O 0.1g / L, TE trace element solution 1mL / L, the rest is water, and the formula of TE solution is H 3 BO 3 2.81g / L, MnCl 2 4H2O 1.82g / L, ZnSO 4 7H2O 0.23g / L, Na 2 MoO 4 2H2O 0.34g / L, CuSO 4 ·5H 2 O 0.05g / L and Co(NO 3 ) 2 ·6H 2 O 0.06g / L; culture temperature 25--28°C, air volume 1.0L / min, add 0.05% organic silicon defoamer; start feeding 1 / 2 Euglena gracilis every day from day 1.5-2 , supplement the same amount of nutrient solution again, and continue to cultivate, wherei...

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Abstract

The invention discloses an euglena gracilis culturing method. The euglena gracilis culturing method comprises the steps of conducting heterotrophic culture on euglena gracilis, discharging part of euglena gracilis in the metaphase of the exponential growth phase, replenishing part of nutrients and water, and continuing culture. The euglena gracilis culturing method has the advantages that high-yield euglena gracilis with a high utilization rate can be cultured quickly, and the production cost of euglena gracilis is reduced greatly.

Description

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Claims

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Application Information

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Owner HENAN NORMAL UNIV
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