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Screening method of culture conditions for high root yield of multiple shoots of bananas

A technology for cultivating conditions and screening methods, applied in horticultural methods, botanical equipment and methods, plant regeneration, etc., can solve the problem of less regulation of banana growth, etc.

Inactive Publication Date: 2017-07-07
黄庆辉
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, banana is a kind of popular fruit, and it is also the main food in some areas. It has a huge market demand. It is necessary to carry out research on the rapid propagation of bananas. It can not only solve the huge market demand, but also be able to To a certain extent, excellent banana varieties are preserved. Tissue culture is one of the methods for rapid propagation of bananas. However, there are few existing banana culture mediums that regulate the growth of bananas by enhancing light sources.

Method used

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Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0018] The present invention will be further described below in conjunction with specific embodiments.

[0019] A kind of screening method of the culture condition of the high rooting rate of banana bushy buds, it may further comprise the steps:

[0020] Step S10 determines the preliminary screening range, retrieves and consults relevant literature, compares the type of enhanced light source added during the rooting process of tissue culture of other plant species and determines the preliminary screening range of the wavelength of the light source;

[0021] Step S20 selects the explant, selects the clustered buds of bananas that are healthy, consistent in the growth environment and in a similar growth state, and divides the clustered buds into single buds;

[0022] Step S30 control culture, with 1 / 2MS medium as the basal medium, the volume fraction of aspirin solution is 0.03%, the concentration of NAA (naphthaleneacetic acid) is 0.15mg / L, PP 333 The concentration of (paclobu...

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Abstract

The invention provides a screening method of culture conditions for a high root yield of multiple shoots of bananas. The method comprises the following steps: determining the optimum enhanced light source wavelength by adjusting the physiological effect of the enhanced light source wavelength on tissue culture seedlings of bananas by employing a single variable method; determining different wavelength light source combinations according to the influence of different wavelength light sources on the tissue culture seedlings of bananas; and determining a basic culture medium reversely by keeping the enhanced light source invariable, wherein at the same time, by introducing the actual root yield of the tissue culture seedlings as a screening criterion, the method is more suitable for commercial industrial production.

Description

technical field [0001] The invention belongs to the technical field of banana tissue culture, and in particular relates to a method for screening the culture conditions with a high rooting rate of banana clustered buds. Background technique [0002] Banana (scientific name: Musa nana Lour.) Musaceae Musa plant, also refers to its fruit. It is widely cultivated and eaten in tropical areas. Savory and nutritious, bananas are harvested year-round and are also valued in temperate regions. The plant is a large herb, emanating from the rhizome, forming a false stem 3-6 meters (10-20 feet) high from the lower part of the leaf sheath; the leaves are oblong to oval, and some are 3-3.5 meters long (10 ~11.5 feet), 65 cm (26 inches) wide, with 10-20 clustered stem tops. drooping spikes [1] , pulled out from the top of the false stem, with many flowers, light yellow; infructescence drooping, resulting in 10-20 clusters, about 50-150. After the plant bears fruit, it dies, and the s...

Claims

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Application Information

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IPC IPC(8): A01H4/00A01G7/04
CPCA01G7/04A01H4/001A01H4/008Y02P60/14
Inventor 不公告发明人
Owner 黄庆辉