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A kind of Microcystis ethanol extract and its preparation method and application

A technology of ethanol and extract of Microcystis, applied in the directions of application, drug combination, pharmaceutical formulation, etc., can solve problems such as few reports, and achieve the effect of significant effect, anti-aging and anti-oxidation effect, and short cycle

Active Publication Date: 2020-01-03
GUANGDONG PHARMA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the research on Microcystis mainly focuses on microcystins, and there are few reports on the biological activity of Microcystis.

Method used

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  • A kind of Microcystis ethanol extract and its preparation method and application
  • A kind of Microcystis ethanol extract and its preparation method and application
  • A kind of Microcystis ethanol extract and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] The preparation of embodiment 1 Microcystis ethanol extract

[0033] S1. Take fresh Microcystis algae, freeze at -30°C for 12 hours, thaw at room temperature for 12 hours, repeat freezing and thawing 4 times, centrifuge at 8000r / min for 10min, and remove the supernatant The solution and the precipitate were separated, and the precipitate was taken for later use;

[0034] S2. Add ethanol with a volume fraction of 85% to the precipitation described in step S1 according to the solid-to-liquid ratio of wet algae and ethanol of 1 g / mL for ultrasonic extraction 3 times, the frequency of ultrasonic extraction is 40 kHz, and the temperature is 4 ° C. The time is 20min, centrifuged at the speed of 8000r / min for 10min, the supernatant and the precipitate are separated, the supernatant is taken for vacuum drying, and the product after vacuum drying is dissolved with first-grade water to adjust its relative The weight concentration of Microcystis (wet algae) is 1gA / mL (A represent...

Embodiment 2

[0036] The preparation of embodiment 2 Microcystis ethanol extract

[0037] S1. Take fresh Microcystis algae, freeze at -20°C for 12 hours, thaw at room temperature for 12 hours, repeat freezing and thawing 5 times, centrifuge at a speed of 10,000r / min for 8min, and remove the supernatant The solution and the precipitate were separated, and the precipitate was taken for later use;

[0038]S2. To the precipitation described in step S1, according to the solid-to-liquid ratio of wet algae and ethanol of 1g / mL, add ethanol with a volume fraction of 70% for ultrasonic extraction for 3 times, the frequency of ultrasonic extraction is 35kHz, and the temperature is 4°C. The time is 30min, centrifuged at the speed of 8000r / min for 10min, the supernatant and the precipitate are separated, the supernatant is taken for vacuum drying, and the product after vacuum drying is dissolved in first-grade water to adjust its relative The weight concentration of Microcystis (wet algae) is 1gA / mL (...

Embodiment 3

[0039] The preparation of embodiment 3 Microcystis ethanol extract

[0040] S1. Take fresh Microcystis algae, freeze at -40°C for 12 hours, thaw at room temperature for 12 hours, repeat freezing and thawing 3 times, centrifuge at 8000r / min for 15min, and remove the supernatant The solution and the precipitate were separated, and the precipitate was taken for later use;

[0041] S2. To the precipitation described in step S1, according to the solid-liquid ratio of wet algae and ethanol 1g / mL, add ethanol with a volume fraction of 90% for ultrasonic extraction 4 times, the frequency of ultrasonic extraction is 40kHz, and the temperature is 4°C. centrifuge for 15 minutes at a speed of 5000r / min, separate the supernatant from the precipitate, take the supernatant for vacuum drying, and dissolve the product after vacuum drying with first-grade water to adjust its relative The weight concentration of Microcystis (wet algae) is 1gA / mL (A represents Microcystis wet algae), and the eth...

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Abstract

The invention belongs to the technical field of marine organisms and provides a microcystis ethanol extract, a preparation method and an application thereof. The microcystis ethanol extract is safe and toxic-free, can promote removal of reactive oxide species (ROS), reduces level of the ROS in body, can alleviate premature senility due to oxidative stress, has significant anti-oxidizing and anti-aging effects, and can be applied to production of health-care foods and medicines having anti-oxidizing effect.

Description

technical field [0001] The invention belongs to the technical field of marine organisms, and in particular relates to an ethanol extract of Microcystis and its preparation method and application. Background technique [0002] Human organisms are composed of many elements, and the level of antioxidant capacity of the human body depends on these constituent elements and their composition. For example, selenium is an essential trace element for living organisms and an important antioxidant in the body, which plays an important role in maintaining human health and delaying aging. Free radicals are normal metabolites of the human body, and free radicals in organisms are mainly oxygen free radicals. Oxygen free radicals are a type of oxygen-containing groups with high chemical reactivity produced by oxygen insufficiency in accepting electrons during reduction, mainly including superoxide free radicals, hydroxyl free radicals, singlet oxygen, peroxyl free radicals and nitrogen per...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K35/748A61P39/06A23L33/135
CPCA23V2002/00A61K35/748A61K2236/10A61K2236/333A61K2236/39A61K2236/51A61K2236/53A23V2200/302A23V2250/206
Inventor 胡丽莉黄泽波卢哲宋立荣余程明张玲屈思雨
Owner GUANGDONG PHARMA UNIV
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