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A method for improving the biological selenium enrichment ability of black soldier flies

A black soldier fly and selenium-enriching technology, which is applied in animal feed, animal husbandry, etc., can solve the problem of low selenium-enrichment biotransformation ability, achieve improved selenium-enrichment biotransformation ability, good competitiveness, and selenium-enriched high level effect

Active Publication Date: 2020-12-04
INST OF ZOOLOGY GUANGDONG ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the low biotransformation ability of black soldier flies for selenium enrichment is the bottleneck of using black soldier flies for selenium enrichment.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Sodium selenite is added in the peanut bran and is formulated with selenium-containing peanut bran (water content 70%), and the concentration of sodium selenite in the selenium-containing peanut bran is respectively 10, 20, 40, 80, 160, 320mg / kg, The 3rd instar larvae of the black soldier fly with the same size were selected for measurement, each group was treated with 30 test worms, repeated 3 times, placed in a greenhouse at 28°C, 60%-80%RH, and the 24h lethal concentration (LC 50 ) is 43.25 mg / kg.

[0018] Black soldier fly eggs are inoculated into peanut bran (water content 70%) and raised, and when the larvae grow to the third instar larvae, they are transferred to selenium-containing peanut bran (water content 70%) containing 43.25mg / kg sodium selenite The pre-pupae were reared to pre-pupae, and the pre-pupae became adults through pupation and eclosion (completed the selection of one generation). After the adults laid eggs, the black soldier fly eggs were inoculat...

Embodiment 2

[0020] According to the method in embodiment 1, measure the 24h LC of the 3rd instar larva of the black soldier fly of the 3rd generation to sodium selenite 50 It is 47.86mg / kg. Black soldier fly eggs are inoculated into peanut bran (water content 70%) and raised, and when the larvae grow to the third instar larvae, they are transferred to selenium-containing peanut bran (water content 70%) containing 47.86mg / kg sodium selenite The pre-pupae were reared to pre-pupae, and the pre-pupae became adults through pupation and eclosion (completed the selection of one generation). After the adults laid eggs, the selection of the next generation was carried out. The selected feeding conditions are 28°C, 60%-80%RH. The selenium-enriched content of the 5th instar larvae and prepupa of the 20th generation was measured using the method for determining selenium in food in GB 5009.93-2010. The results showed that the selenium content of the 5th instar larvae and prepupa (wet weight) was 51.2...

Embodiment 3

[0023] Sodium selenate is added in peanut bran and is mixed with selenium-containing peanut bran (water content 50%). The 3rd instar larvae of the same black soldier fly were measured, each group was treated with 30 test worms, repeated 3 times, placed in a greenhouse at 28°C, 60%-80%RH, and the 24h LC was measured 50 It was 49.11mg / kg.

[0024] Black soldier fly ovum is inoculated into peanut bran (water content 50%) and is raised, and when the larva grows to the third instar larvae, it is transferred to the selenium-containing peanut bran (water content 50%) containing 49.11mg / kg sodium selenate Breeding until the pre-pupae, the pre-pupae become adults after pupation and eclosion (complete the selection of one generation), and after the adults lay eggs, the selection of the next generation is carried out, and 20 generations are selected in this way. The conditions are 28° C., 60% to 80% RH. The selenium-enriched content of the 5th instar larvae and prepupa of the 20th gene...

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Abstract

The invention discloses a method for raising blackwater gadfly bio-selenium capability, and the method comprises the steps of inoculating blackwater gadfly eggs to a forage for breeding; when the larva is 3 years old, taking an inorganic selenium forage that has a median lethal concentration for 3-years larva as a resistance-selection forge, and inoculating the 3-years larva to the resistance-selection forge for breeding until prepupa. The larva becomes mature after pupation and feathering, and then lays eggs to complete the resistance-selection of the first generation. The steps are repeated to get into the resistance-selection of the next generation. The resistance-selection in cycle is conducted for at least 3 generations. According to the invention, the capability of selenium-enrichment biological conversion for the blackwater gadfly is raised; the bottleneck that existing blackwater gadfly has low selenium enrichment is solved; and the selenium enrichment of the blackwater gadfly after resistance-selection is high. In this way, the selenium-rich blackwater gadfly can be used as an excellent additive to produce selenium-rich foodstuff and medicine; good market competitiveness is provided; new entelechy and vitality in functional foodstuff industry in our country is provided; at the same time, the added value of the blackwater gadfly is raized.

Description

Technical field: [0001] The invention belongs to the technical field of biological selenium enrichment, and in particular relates to a method for improving the biological selenium enrichment ability of black soldier flies. Background technique: [0002] Selenium (Selenium) is an oxygen group non-metallic element. Since 1817, Berzelius of Sweden has produced selenium from the red powder substance at the bottom of the lead chamber of the sulfuric acid plant. Selenium has important functions such as anti-oxidation, anti-cancer, improving human immunity, preventing cardiovascular diseases, antagonism, and resisting the toxicity of harmful metal elements in the human body. If the human body lacks selenium, it is easy to suffer from Kaschin-Beck disease, Keshan disease, gastric cancer, etc.; selenium also has the effect of weakening the effect of aflatoxin on liver cancer and inhibiting the occurrence of breast cancer. At present, the problem of insufficient selenium intake is co...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K67/033A23K50/90
CPCA01K67/033
Inventor 徐齐云安新城韩诗畴
Owner INST OF ZOOLOGY GUANGDONG ACAD OF SCI
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