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Preparation method and application of pittosporum tobira fruit extract

An extract and fruit technology, which is applied in the field of preparation of Pittosporum fruit extracts, can solve the research reports on the analysis of volatile chemical components of the Pittosporum fruit extracts, the research reports on the anti-endotoxin effects, etc. that have not been seen yet. problem, to achieve the effect of simple and reasonable design

Inactive Publication Date: 2017-07-25
HUAIHAI INST OF TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

So far, ① there is no research report on the analysis and research on the volatile chemical components of Pittosporum fruit extract;

Method used

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  • Preparation method and application of pittosporum tobira fruit extract
  • Preparation method and application of pittosporum tobira fruit extract
  • Preparation method and application of pittosporum tobira fruit extract

Examples

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preparation example Construction

[0015] A preparation method of Pittosporum fruit extract, the steps are as follows:

[0016] (1) take Pittosporum fruit, the Pittosporum fruit that takes is mixed with 95% ethanol;

[0017] (2) reflux extraction and suction filtration at high temperature, collect the extract, and extract the filter residue 3 times, and combine the extract;

[0018] (3) Concentrating the extract in step (2) under reduced pressure in vacuo and drying to constant weight to obtain Pittosporum fruit extract.

[0019] The fruit extract of Pittosporum was ultrasonicated with methanol, and its supernatant was analyzed by gas chromatography-mass spectrometry (GC-MS), and the following analysis results were obtained.

[0020] The total ion chromatogram of GC-MS analysis is as follows:

[0021] ① The chromatographic conditions are as follows:

[0022] Chromatographic column: DB-5MS, 30m*0.25mm*0.25um, carrier gas He 1.0ml / min. Column temperature: 40°C*3min---10°C / min---100°C---6° / min---300°C*5min

...

Embodiment 1

[0042] A preparation method of Pittosporum fruit extract, characterized in that the steps are as follows:

[0043] (1) Take Pittosporum fruit 800g, the Pittosporum fruit that takes is mixed with 95% ethanol with 1:3 weight ratio;

[0044] (2) reflux extraction at a temperature of 80 degrees Celsius for 3 hours, suction filtration, collecting the extract, and extracting the filter residue 3 times, and combining the extract;

[0045] (3) Concentrating the extract in step (2) under reduced pressure in vacuo and drying to constant weight to obtain Pittosporum fruit extract.

[0046] Pittosporum fruit extract is an active ingredient that can prepare antitoxin.

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Abstract

The invention relates to a preparation method of pittosporum tobira fruit extract. The preparation method is characterized by comprising the following steps of: (1) weighing pittosporum tobira fruits, and mixing the weighed pittosporum tobira fruits with 95% ethanol; (2) performing reflux extraction and suction filtration at a high temperature, collecting an extraction liquid, extracting the filter residues for three times, and combining the extraction liquid; and (3) concentrating the extraction liquid in the step (2) in vacuum at a reduced pressure and drying the same to constant weight to obtain the pittosporum tobira fruit extract. The preparation method provided by the invention is simple and reasonable in design, and can effectively extract active ingredients of the pittosporum tobira fruits. In important raw materials such as medicines, food and cosmetics, more than half of the compounds are sesquiterpenoids which almost have relatively strong fragrance and biological activity, have many important biological functions and physiological activity and further have activities such as bacteria resistance, virus resistance, cytokine, immunosuppression, tumor resistance, phytotoxin, insect hormone and insect anti-feedant and etc.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a preparation method and application of Pittosporum fruit extract. Background technique [0002] Pittosporum tobira (scientific name: Pittosporum tobira), the genus Pittosporum tobira. It grows in coastal areas such as Guangdong, Hainan, Fujian, and Zhejiang, and is a traditional Chinese medicinal material in my country. The fruit of Pittosporum can be used as medicine, can astringe intestines, has certain curative effect on jaundice and nephritis, and has the effect of reducing inflammation and swelling, and relieving cough and asthma. Studies have shown that Pittosporum fruit contains triterpenes and carotenoids, and contains a lot of palmitic acid and oleic acid. So far, ① there is no research report on the analysis and research of the volatile chemical components of Pittosporum fruit extract; ② there is no research report on the anti-endotoxin effect of the volatile...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C7/00C07C7/10C07C13/605A61K31/015A61P39/02
CPCC07C7/005A61K31/015A61K36/48C07C7/10C07C13/605
Inventor 宋晓凯张秀莲
Owner HUAIHAI INST OF TECH
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