Compound microbial agent for riverway treatment and preparation method and application thereof
A technology of compound microbial inoculants and microbial inoculants, applied in the field of environmental microorganisms, can solve problems such as affecting the effect of pollution control, staying in the laboratory stage, and secondary pollution of water bodies.
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Embodiment 1
[0039] Preparation of composite microbial agent for in-situ remediation of urban and rural river sewage
[0040] (1) Preparation of composite microbial bacterial agent A:
[0041] (1.1) Take the seeds of Bacillus megaterium CCTCC No.M2012352, inoculate them in solid slant medium I for activation culture, the activation conditions are: 32°C, activate the culture for 48h; then inoculate the activated Bacillus megaterium CCTCC No.M2012352 into liquid culture The base I was expanded and cultivated, and the expanded culture conditions were as follows: in a rotary shaker at 200 rpm, cultivated at 30°C for 48 hours; the expanded cultured Bacillus megaterium CCTCC No.M2012352 was inoculated in the fermentation medium I by 10% of the volume of the fermentation broth , under the rotation speed of 150rpm in a rotary shaker, after culturing at 32°C for 96h, the concentration of Bacillus megaterium CCTCC No.M2012352 in the obtained fermentation liquid of Bacillus megaterium CCTCC No.M20123...
Embodiment 2
[0071] Application of composite microbial agent for in-situ remediation of urban and rural river sewage
[0072]A certain river in the suburbs of Tongdao County, Huaihua, Hunan Province, has urban domestic sewage, rural breeding and planting sewage discharges, and mountain floods all year round. The river water is turbid and smelly all the year round, and COD, ammonia nitrogen, and total phosphorus exceed the standard. Get 2L water sample from this river course, add 2mL of the liquid compound microbial bacterial agent A that embodiment prepares, add bacterial agent once every three days, after feeding continuously for three weeks, measurement result shows, water body COD is by 260mg / L before putting in microorganism decreased to 14.0mg / L; ammonia nitrogen content decreased from 17.2mg / L before restoration to 0.66mg / L; total nitrogen decreased from 32.4mg / L to 0.91; total phosphorus decreased from 2.80mg / L to 0.17; add another 20mL to implement For the liquid microbial agent B ...
Embodiment 3
[0076] Application of composite microbial agent for in-situ remediation of urban and rural river sewage
[0077] A certain river in Shimen County, Changde, Hunan, is turbid all year round, and COD, ammonia nitrogen and total phosphorus exceed the standard. Get 2L water samples from the river, add 2.5mL of the liquid composite microbial agent A prepared by the embodiment, add the agent once every four days, after three weeks of continuous feeding, the measurement results show that the COD of the water body is changed from 245mg / L decreased to 12.6mg / L; ammonia nitrogen content decreased from 19.2mg / L before restoration to 0.62mg / L; total nitrogen decreased from 35.24mg / L to 0.95; total phosphorus decreased from 3.03mg / L to 0.14; For the liquid microbial agent B prepared in Example 1, the turbidity and chromaticity of the water quality improved significantly after being added for 3 days, and the transparency increased from 15cm to 27cm, and the transparency increased to 68cm af...
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