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Fusarium sp. strain and application thereof

A technology of Fusarium, strains, applied in the field of bioengineering

Active Publication Date: 2017-08-01
GUANGZHOU UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The present invention aims at the above-mentioned technical defects of using existing methods to deal with heavy metal thallium pollution, and provides a Fusarium sp. Remedial treatment of heavy metal thallium pollution

Method used

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  • Fusarium sp. strain and application thereof
  • Fusarium sp. strain and application thereof
  • Fusarium sp. strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Embodiment 1: Isolation and identification of FP-JCCW bacterial strain

[0039] (1) The FP-JCCW strain is a resistant strain screened from the Shaoguan Dabaoshan mining area, which specifically includes the following steps:

[0040] (1) Prepare culture medium

[0041] PDA medium: potato (peeled) 200g, glucose 20g, agar 20g, distilled water 1000mL, natural pH value.

[0042] The specific preparation process of PDA medium: Peel 200g of potatoes and bud eyes, cut into small pieces, put them in 1000mL of distilled water and boil until the potatoes become soft and not rotten, then filter with 8 layers of gauze, and pour the filtrate back into In the pot, add agar and glucose, stir evenly and replenish water to 1000mL, and finally divide into 250mL Erlenmeyer flasks for later use.

[0043] (2) Domestication and cultivation

[0044] Make a spore suspension from freshly cultured endophytic fungal strains to the logarithmic phase, take 1mL of the spore suspension and apply it...

Embodiment 2

[0052] Example 2: Performance and mechanism of action of FP-JCCW strain for adsorption of heavy metal thallium

[0053] In order to further clarify the adsorption characteristics and preliminary mechanism of FP-JCCW strains on heavy metal thallium, this example takes thallium as the research object, and studies the adsorption characteristics of microbial strains on thallium under different environmental conditions, using electron microscopy, infrared spectroscopy and other methods to study Preliminary mechanism of bacterial strain adsorption of heavy metal thallium.

[0054] (1) Preparation of FP-JCCW strain adsorbent

[0055] The FP-JCCW strain was cultured in liquid for 72 hours and filtered through 8 layers of gauze to obtain FP-JCCW mycelium. Then the cells were transferred to an enamel dish, and placed in an oven at 80°C for 6 hours. The dried mycelium was placed in a mortar and ground into powder to obtain the inactivated microorganism FP-JCCW (adsorbent).

[0056] Th...

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Abstract

The invention belongs to the technical field of biological engineering, and particularly discloses a Fusarium sp. strain and application thereof. The Fusarium sp. strain is identified as Fusarium sp. by an ITS gene sequence, and has a preservation number of CCTCC M 2016582. An inactivated FP-JCCW thallus is used for researching the adsorption behavior of the FP-JCCW thallus to thallium, thus obtaining the following optimal absorption conditions: pH is 5.0, initial concentration is 100mg / L, contact time is 90min, revolving speed is 150r / min, and biomass is 2.5g / L; the adsorption more accords with a Langmuir Isothermal model and a second-order kinetic equation, belongs to single molecular layer adsorption, and takes chemical adsorption as the principal. Shown by FT-IR, SEM and XRD characterization analysis, FP-JCCW is porous on the surface, the adsorption of the FP-JCCW to the thallium mainly relies on hydroxyl, carbonyl and the like on cell walls, and a crystal structure of the thallus is changed after adsorption; deduced thereby, the adsorption of the FP-JCCW to the thallium is performed by electrostatic adsorption and surface complexation. The inactivated microorganism FP-JCCW can serve as an economical, environment-friendly and sustainable adsorbent to treat heavy metal thallium pollution.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, relates to microorganisms and applications thereof, in particular to a Fusarium sp. strain FP-JCCW, and the application of the inactivated microorganisms as adsorbents in the treatment of heavy metal thallium pollution. Background technique [0002] With the development of industrialization, the problem of heavy metal treatment after large-scale mining has become increasingly prominent. The heavy metal thallium is highly toxic and easily enriched in sulfides. It often migrates during mining and can enter the human body along the food chain, posing a potential threat to human life. [0003] For mining, especially thallium ore and pyrite, the effective treatment of heavy metals is the key to the sustainable development of the mining industry. Nowadays, the treatment of heavy metals is mostly based on physical and chemical methods, but compared with microbial treatment, the two have defects ...

Claims

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Application Information

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IPC IPC(8): C12N1/14C02F3/34C12R1/77C02F101/20
CPCC02F3/34C02F2101/20C12N1/145C12R2001/77
Inventor 龙建友陈永亨陈迪云郑邦丰罗定贵蔡倩怡
Owner GUANGZHOU UNIVERSITY
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