Method for conducting fixation of denitrifying bacteria with Trichodermaviride as carrier

A technology of denitrifying bacteria and Trichoderma viride, applied in the field of environmental bioengineering and water treatment, can solve problems affecting the ability of denitrification, achieve the effects of improving water quality, reducing environmental pollution, and reducing nitrogen content

Active Publication Date: 2017-08-18
QUFU NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, immobilizing denitrifying bacteria on these chemical carriers often inhibits the activity of the ba

Method used

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  • Method for conducting fixation of denitrifying bacteria with Trichodermaviride as carrier
  • Method for conducting fixation of denitrifying bacteria with Trichodermaviride as carrier
  • Method for conducting fixation of denitrifying bacteria with Trichodermaviride as carrier

Examples

Experimental program
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Effect test

Embodiment 1

[0041] A method for immobilizing denitrifying bacteria with Trichoderma viride as a carrier is obtained by the following steps:

[0042] 1) Take two rings of paracocci from the inclined surface and inoculate them into the enrichment medium, cultivate them on a shaking table, centrifuge the culture medium to remove the supernatant, and obtain bacteria; suspend the bacteria in sterile normal saline, and obtain denitrifying bacteria suspensions. liquid;

[0043] In the step 1), the concentration of sterile normal saline is 0.9%; the mass ratio of bacteria to sterile normal saline is 1:50.

[0044] In the step 1), the composition of the enrichment medium is as follows: KNO 3 10g / L, peptone 0.1g / L, beef extract 20g / L, K 2 HPO 4 ·3H 2 O 0.1g / L, MgSO 4 ·7H 2 O 5g / L, the balance is water.

[0045] In the step 1), the rotation speed of the shaker culture is 100r / min, the culture temperature is 35°C, and the culture time is 12h.

[0046] 2) Take two rings of Trichoderma viride fro...

Embodiment 2

[0059] A method for immobilizing denitrifying bacteria with Trichoderma viride as a carrier is obtained by the following steps:

[0060] 1) Take two rings of paracocci from the inclined surface and inoculate them into the enrichment medium, cultivate them on a shaking table, centrifuge the culture medium to remove the supernatant, and obtain bacteria; suspend the bacteria in sterile normal saline, and obtain denitrifying bacteria suspensions. liquid;

[0061] In the step 1), the concentration of sterile normal saline is 0.9%; the mass ratio of bacteria to sterile normal saline is 1:150.

[0062] In the step 1), the composition of the enrichment medium is as follows: KNO 3 5g / L, peptone 10g / L, beef extract 10g / L, K 2 HPO 4 ·3H 2 O 3g / L, MgSO 4 ·7H 2 O 3g / L, the balance is water.

[0063] In the step 1), the rotation speed of the shaker culture is 150r / min, the culture temperature is 25°C, and the culture time is 48h.

[0064] 2) Take two rings of Trichoderma viride from...

Embodiment 3

[0077] A method for immobilizing denitrifying bacteria with Trichoderma viride as a carrier is obtained by the following steps:

[0078] 1) Take two rings of paracocci from the inclined surface and inoculate them into the enrichment medium, cultivate them on a shaking table, centrifuge the culture medium to remove the supernatant, and obtain bacteria; suspend the bacteria in sterile normal saline, and obtain denitrifying bacteria suspensions. liquid;

[0079] In the step 1), the concentration of sterile physiological saline is 0.9%; the mass ratio of bacteria to sterile physiological saline is 1:250.

[0080] In the step 1), the composition of the enrichment medium is as follows: KNO 3 0.1g / L, peptone 20g / L, beef extract 0.1g / L, K 2 HPO 4 ·3H 2 O5g / L, MgSO 4 ·7H 2 O 0.1g / L, the balance is water.

[0081] In the step 1), the rotation speed of the shaker culture was 200r / min, the culture temperature was 19°C, and the culture time was 72h.

[0082] 2) Take two rings of Tr...

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Abstract

The invention discloses a method for conducting fixation of denitrifying bacteria with Trichodermaviride as a carrier. The method includes the following steps that Paracoccussp. is inoculated to an enrichment medium, shaking culture is conducted, the obtained bacteria are suspended in a sterile saline solution, and a bacterium suspension of the denitrifying bacteria is obtained; Trichodermaviride is inoculated to a solid medium for culture, washing is conducted with sterile water, the cultured Trichodermaviride is stored at low temperature, and a spore suspension is obtained; the bacterium suspension of the denitrifying bacteria and the spore suspension are inoculated to a mycelium pellet medium to conduct shaking culture, and mixed mycelium pellets of fixation of denitrifying bacteria with Trichodermaviride as the carrier are obtained. The method has the advantages of being simple and convenient in operation, short in culture time, reusable and good in sedimentation performance, the mixed mycelium pellets prepared can obviously reduce the nitrogen content of a water body, water quality conditions are improved, and sewage reaches the discharge standard; nitrite accumulation does not exist, environmental pollution is reduced, and the method has important significance for sewage treatment by means of Paracoccussp. on a large scale.

Description

technical field [0001] The invention belongs to the fields of environmental biological engineering and water treatment, and in particular relates to a method for immobilizing denitrifying bacteria with Trichoderma viride as a carrier. Background technique [0002] With the development of industrial and agricultural production and the improvement of people's living standards, the discharge of nitrogen-containing pollutants has increased rapidly, and the problem of nitrogen pollution in the water environment has become increasingly serious, becoming a major problem facing the international community. Nitrate nitrogen and nitrite nitrogen are the most serious nitrogen pollution in domestic water bodies, and biological nitrogen removal is one of the most effective methods to remove nitrogen pollution in water bodies. [0003] Biological denitrification technology usually consists of nitrification process and denitrification process. The nitrification process is mainly to remove...

Claims

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Application Information

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IPC IPC(8): C12N11/16C02F3/34C12R1/885C12R1/01C02F101/16
CPCC02F3/347C02F2101/163C12N11/16
Inventor 杨革刘胜格巩志金车程川刘金锋
Owner QUFU NORMAL UNIV
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