Preparation method of surface plasma resonance instrument chip based on modifying of amphoteric ion polymethyl allyl cysteine

A technology based on polymethacryl and methacryl, which is applied in the field of anti-pollution chip preparation for ion resonance spectrometers, can solve the problems of reducing the accuracy of quantitative detection, surface contamination of sensor chips, false positives, etc., and achieve anti-pollution performance No change, high stability, good anti-pollution performance

Active Publication Date: 2017-08-18
津海威视技术(天津)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, when surface plasmon resonance is used for analysis and testing, the surface contamination of the sensor chip is a common problem, and non-spe

Method used

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  • Preparation method of surface plasma resonance instrument chip based on modifying of amphoteric ion polymethyl allyl cysteine
  • Preparation method of surface plasma resonance instrument chip based on modifying of amphoteric ion polymethyl allyl cysteine
  • Preparation method of surface plasma resonance instrument chip based on modifying of amphoteric ion polymethyl allyl cysteine

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] A 2nm thick chromium layer is coated on the BK7 glass substrate by electron beam evaporation coating technology, and a 48nm thick gold film is coated on the chromium layer to obtain a bare gold chip; the above bare gold chip is immersed in 98% concentrated In the mixed solution of sulfuric acid and 30% hydrogen peroxide, the volume ratio of 98% concentrated sulfuric acid to 30% hydrogen peroxide is 7:3, soak at room temperature for 2 hours. Then take it out and wash it with deionized water for 3 times, then dry it with nitrogen gas for later use.

[0035] After dissolving 24.98mmol of cysteine ​​in 20mL of deionized water, add 27.47mmol of 2-acrylic acid-2-hydroxy-1,3-propylene glycol and 29.4μmol of xylylphosphine, and stir the reaction at 20°C for 4h , the reacted solution was extracted once with ethyl acetate, extracted twice with dichloromethane, and freeze-dried to obtain a white powdery zwitterionic methacryl cysteine ​​monomer;

[0036] Prepare a 2 mg / mL dopamin...

Embodiment 2

[0039]A 3nm thick chromium layer is coated on the BK7 glass substrate by electron beam evaporation coating technology, and a 47nm thick gold film is coated on the chromium layer to obtain a bare gold chip; the above bare gold chip is immersed in 98% concentration In the mixed solution of sulfuric acid and 30% hydrogen peroxide, the volume ratio of 98% concentrated sulfuric acid to 30% hydrogen peroxide is 7:3, soak at room temperature for 1 hour. Then take it out and wash it with deionized water for 3 times, then dry it with nitrogen gas for later use.

[0040] After dissolving 24.98mmol of cysteine ​​in 20mL of deionized water, add 27.47mmol of 2-acrylic acid-2-hydroxy-1,3-propylene glycol and 29.4μmol of xylylphosphine, and stir the reaction at 30°C for 2h , the reacted solution was extracted once with ethyl acetate, extracted twice with dichloromethane, and freeze-dried to obtain a white powdery zwitterionic methacryl cysteine ​​monomer;

[0041] Prepare a 3 mg / mL dopamine...

Embodiment 3

[0044] A 2nm thick chromium layer is coated on the BK7 glass substrate by electron beam evaporation coating technology, and a 48nm thick gold film is coated on the chromium layer to obtain a bare gold chip; the above bare gold chip is immersed in 98% concentrated In the mixed solution of sulfuric acid and 30% hydrogen peroxide (volume ratio 7:3), the volume ratio of 98% concentrated sulfuric acid to 30% hydrogen peroxide is 7:3, soak at room temperature for 2 hours. Then take it out and wash it with deionized water for 3 times, then dry it with nitrogen gas for later use.

[0045] After dissolving 24.98mmol of cysteine ​​in 20mL of deionized water, add 27.47mmol of 2-acrylic acid-2-hydroxy-1,3-propylene glycol and 29.4μmol of xylylphosphine, and stir the reaction at 20°C for 4h , the reacted solution was extracted once with ethyl acetate, extracted twice with dichloromethane, and freeze-dried to obtain a white powdery zwitterionic methacryl cysteine ​​monomer;

[0046] Prepar...

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Abstract

The invention discloses a preparation method of a surface plasma resonance instrument chip based on modifying of amphoteric ion polymethyl allyl cysteine. The preparation method comprises the following steps of obtaining a methyl allyl cysteine monomer through sulfhydryl-alkene clicking reaction; modifying an initiator to the surface by polydopamine at the surface of a gold film; using a surface-induced atom transfer free radical polymerizing method to initiate the monomer to polymerize at the surface of the gold film, so as to obtain the surface plasma resonance instrument chip based on the modifying of the amphoteric ion polymethyl allyl cysteine. The chip has strong anti-pollution property on a single protein solution and a natural complicated sample system, and has high solid carrying amount and good compatibility on biological macromolecules, such as antibody, and realizes high-sensitive and non-marker quick detection on target molecules in a complicated protein system. The preparation method has the advantages that the anti-pollution property is strong, the stability is good, the solid carrying amount is high, and the like; the feasibility and practicality are good.

Description

technical field [0001] The invention relates to the field of preparation of an anti-pollution chip for a surface plasmon resonance spectrometer, in particular to a preparation method for a surface plasmon resonance spectrometer chip based on zwitterionic polymethylacrylcysteine ​​modification. Background technique [0002] The Surface Plasmon Resonance (SPR) instrument is a biosensor technology that appeared in the 1980s. When the incident light is incident from a high-refractive-index medium to a low-refractive-index medium, total reflection will occur. A layer of metal film (gold or silver) is coated at the interface of the medium. The evanescent wave generated by the incident light will cause the free electrons on the metal surface to oscillate collectively, and then form plasma. When the frequency and wave number of the evanescent wave and surface plasmon oscillation When they are equal, the resonance phenomenon (SPR) will occur, which will cause the energy to transfer f...

Claims

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Application Information

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IPC IPC(8): G01N21/552
CPCG01N21/553
Inventor 丁利苏荣欣叶慧君王利兵齐崴王季诚
Owner 津海威视技术(天津)有限公司
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