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A method for identifying buckwheat black spot resistance

A buckwheat black spot and resistance technology, which is applied in the field of crop disease and insect resistance identification, can solve problems such as restricting the selection and breeding of buckwheat black spot disease-resistant varieties, and achieve the effects of short detection period, simple operation and low cost.

Active Publication Date: 2020-09-04
云南省农业科学院生物技术与种质资源研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are no identification methods of buckwheat black spot resistance and disease-resistant buckwheat germplasm resources in China, which seriously restricts the breeding of buckwheat black spot disease-resistant varieties. Sex identification method is imminent

Method used

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  • A method for identifying buckwheat black spot resistance
  • A method for identifying buckwheat black spot resistance
  • A method for identifying buckwheat black spot resistance

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] This embodiment relates to the specific evaluation examples of buckwheat black spot resistance:

[0056] In the experiment, the variety Mihei 3 with high resistance, Zongku 2 with moderate resistance, YNK7 with moderate sensitivity and YNK43 with high sensitivity were used as test materials.

[0057] Buckwheat seeds were provided by the Germplasm Resources Preservation Center of Yunnan Academy of Agricultural Sciences.

[0058] Strain and source of black spot pathogen: the highly pathogenic strain HN-3 of black spot pathogen was isolated from a sample in Anning, Yunnan Province by the buckwheat pathology laboratory of our laboratory.

[0059] Specific steps:

[0060] (1) Transplant the bacterial classification of buckwheat black spot pathogen to the PDA medium plate for activation and culture for 7 days; scrape the culture, and prepare it with a concentration of 1×10 6 CFU / mL spore suspension, set aside.

[0061] (2) At the 4-5 leaf stage of buckwheat plants, take fr...

Embodiment 2

[0084] Using the method described in 1, Mihei 3, Ningqiao 1, Zongku 2, Meng 0353, YNK43, Yunqiao 1, and YNK7 were identified for black spot resistance. One experimenter collected the healthy leaves of the above seven different buckwheat varieties, and numbered the seven different varieties with Arabic numerals, then handed over the numbered leaves to another experimenter, and carried out the experiment according to the steps of method 1. After the experiment is over, check the variety name against the serial number. The results showed that the resistance performance of seven different buckwheat varieties to black spot was significantly different, and the resistance results of Mihei 3, Zongku 2, YNK7 and YNK43 were consistent with the actual performance in the field. The verification test of resistance identification confirmed again that the results of resistance identification were consistent with the known actual situation. Therefore, it was verified that this method can be ...

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Abstract

The invention relates to a method for identifying the buckwheat black spot resistance. The method comprises the following steps that 1, healthy buckwheat leaf surfaces are treated with sterile water, a syringe needle is used for puncturing the front faces of the leaves, and a black spot pathogenic bacterium spore suspension is inoculated on an acupuncture point to obtain inoculated leaves; 2, the inoculated leaves are cultured under the constant temperature condition, and the sizes of scabs is measured after culture is completed; 3, the sizes of the scabs are classified, the disease indexes of the leaves are calculated according to the grades, and resistance evaluation is performed. The method is easy to implement, short in period, accurate and reliable in evaluated result and suitable for large-scale application and popularization.

Description

technical field [0001] The invention relates to the field of identifying resistance to crop diseases and insect pests, in particular to a method for identifying resistance to buckwheat black spot disease. Background technique [0002] Buckwheat black spot is an important fungal disease caused by Alternaria alternata, which is widely distributed in buckwheat planting areas in my country, and the incidence rate can be as high as 65-80% when the damage is serious. The disease mainly infects the leaves of buckwheat, causing the leaves to turn yellow and prematurely senile, and the plants grow slowly, leading to a serious decline in yield and quality, and causing serious economic losses to buckwheat production. The occurrence and damage of buckwheat black spot has become one of the main factors seriously affecting the high yield and quality of buckwheat in my country. Therefore, the scientific and effective prevention and control of buckwheat black spot will become the key to im...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/18G06Q50/02C12R1/645
CPCC12Q1/18G06Q50/02
Inventor 卢文洁王莉花王艳青尹桂芳孙道旺李春花
Owner 云南省农业科学院生物技术与种质资源研究所