Novel micropipettor sucker

Abstract

The invention provides a novel micropipettor sucker, and belongs to the technical field of injection operations of a substrate in the measurement of enzymatic kinetics. The sucker comprises a tubular structure and an inverted cone shaped sucker body, wherein openings are formed in the two ends of the inverted cone shaped sucker body; a micropipettor connection port is formed in the end of the sucker body, where the diameter of the opening is larger; a liquid suction port is formed in the end of the sucker body, where the diameter of the opening is smaller; spraying holes are formed in the side wall of the sucker body. A plurality of layers of spraying holes are formed in the sucker of a micropipettor, and the substrate is synchronously and evenly distributed in an upper layer, a middle layer and a lower layer of a catalytic system solution through one injection. Moreover, the spraying holes are tangent to the tube wall of the sucker, a substrate solution is sprayed out at a high speed from the tangent direction, the novel micropipettor sucker has a certain stirring function, the capability of rapidly and evenly distributing the substrate in a catalytic system is further improved, additionally stirring or turning a cuvette manually is not needed, so that the novel micropipettor sucker is favorable for the reliability, accuracy and repeatability of kinetics measurement data, and the accidental error is reduced.

Description

technical field [0001] The invention belongs to the technical field of enzymatic kinetic determination substrate injection operation, in particular to a novel micropipette tip. Background technique [0002] In enzyme kinetic or enzyme-mimicking catalytic kinetic assays, each kinetic assay begins by injecting the required volume of substrate solution into the catalytic system using a micropipette. The distribution state and uniformity of the substrate in the catalytic system during injection will affect the accuracy of each kinetic measurement result, especially for reactions with fast reaction rates, more attention should be paid to the rapidity of the substrate in the catalytic system Evenly distributed. At present, the tips used for pipetting solutions with micropipettes on the market all use the front of the tip as the only ejection channel for the solution. In the actual operation of pipetting and injecting the substrate solution into the catalytic system, in order to a...

AI Technical Summary

Problems solved by technology

However, this redundant manual stirring operation or inversion operation will cause the delay time to be too long, causing obvious errors in the kinetic determination

At the same time, tiny air bubbles may appear during stirring or inversion, and the optical path changes caused by the uneven density of the medium when the optical path passes through the solution to be tested.

Because the traditional micropipette tip only has the only ejection outlet at the tip, it is bound to have the above-mentioned problems that need to be solved urgently

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