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Molecular marker CD71 and applications in diagnosis, staging and prognosis of endometrial carcinoma

An endometrial cancer, molecular marker technology, applied in the field of molecular biology, can solve the problems of no expression, low expression, and no transferrin receptor yet

Inactive Publication Date: 2017-08-29
INSITUTE OF BIOPHYSICS CHINESE ACADEMY OF SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Transferrin receptors (TfR1, CD71) are highly expressed in actively proliferating and dividing cells, especially on the surface of malignant tumor cells that require a large amount of iron and some rapidly dividing cells. These receptors are not expressed or extremely expressed in normal tissues. Low expression, studies have shown that transferrin receptor (CD71) is highly expressed in a variety of malignant tumors, such as liver cancer, breast cancer, brain malignant glioma, etc. At present, there is no transferrin receptor (TfR1, CD71) in human Research reports on the expression of endometrial cancer tissue, and there is no data on the use of CD71 for clinical prognosis prediction

Method used

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  • Molecular marker CD71 and applications in diagnosis, staging and prognosis of endometrial carcinoma
  • Molecular marker CD71 and applications in diagnosis, staging and prognosis of endometrial carcinoma
  • Molecular marker CD71 and applications in diagnosis, staging and prognosis of endometrial carcinoma

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Experimental program
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Effect test

Embodiment 1

[0027] Immunohistochemical detection and comparison of transferrin receptor expression in normal endometrial tissue, simple hyperplasia endometrial tissue and endometrial cancer tissue.

[0028] 1. Experimental materials

[0029] Tissue microarray (TMA) was purchased from Shanghai Zhuoli Biotechnology Co., Ltd., including 57 samples of endometrial cancer, 23 samples of normal endometrium, and 7 samples of simple hyperplasia endometrium. Immunohistochemical reagents: Anti-human transferrin receptor antibody (EC-1) was prepared by conventional mouse hybridoma method (Edward A. Greenfield. Generating Monoclonal Antibodies. Antibodies: A Laboratory Manual, Second Edition, 2014, Cold Spring Harbor Laboratory Press), details can be found, the contents of which are incorporated herein by reference.

[0030] 1. Experimental method

[0031] Routine immunohistochemical SP method. (De Jong AS, Van Kessel-van Vark M, RaapAK.Histochem J.1985Oct; 17(10):1119-30.Sensitivity of variousvisu...

Embodiment 2

[0051] Immunohistochemical detection of transferrin receptor expression in endometrial carcinoma tissues of different clinical stages.

[0052] 1. Experimental materials

[0053] Tissue microarray (TMA) was purchased from Shaanxi Alina Biotechnology Co., Ltd., according to FIGO staging, including 158 FIGO stage 1 specimens, 32 specimens at stage 2, 29 specimens at stage 3, and 4 specimens at stage 4. Immunohistochemical reagents: anti-human transferrin receptor antibody was EC-1 as described previously.

[0054] 2. Experimental method: with embodiment 1.

[0055] 3. Experimental results:

[0056] Endometrial cancer tissues of different clinical stages (FIGO stages) are correlated with the expression of anti-human transferrin receptor. Chi-square test showed: p Figure 4 )

[0057] 4 Conclusion

[0058] The higher the clinical stage of endometrial cancer and the worse the prognosis, the stronger the expression of anti-human transferrin receptor. Thus, the clinical stage an...

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Abstract

The present invention relates to a molecular marker CD71 and applications in diagnosis, staging and prognosis of endometrial carcinoma, and belongs to the field of molecular biology, wherein the molecular marker is human transferrin receptor 1 (CD71). According to the present invention, by using the anti-human transferrin receptor 1 (CD71) antibody, the results show that human transferrin receptor 1 (CD71) is not expressed or extremely little expressed in normal endometrial tissue and precancerous lesion tissue, is specifically and highly expressed in human endometrial carcinoma tissue, and is related to the staging and the prognosis of endometrial carcinoma, such that CD71 can be used in the diagnosis, the clinical staging and the prognosis of endometrial carcinoma tissue.

Description

technical field [0001] The invention relates to a molecular marker, the application of an anti-human transferrin receptor (transferrin receptor 1, TfR1, also known as CD71) antibody in the diagnosis, clinical staging and prognosis evaluation of endometrial cancer, which belongs to the field of molecular biology. Background technique [0002] Endometrial cancer is one of the three common malignant tumors of the female reproductive system, accounting for about 7% of female cancers and 20-30% of female reproductive system malignant tumors. In recent years, the incidence of endometrial cancer has been on the rise worldwide, and it is younger (Parkin DM, Bray F, Ferlay J, et al. Global cancer statistics, 2002[J]. CA Cancer J Clin, 2005 , 55(2):74-108). [0003] With the in-depth study of traditional markers and the continuous discovery of new markers, immunohistochemistry plays an increasingly important role in the pathological diagnosis and differential diagnosis of endometrial...

Claims

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Application Information

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IPC IPC(8): G01N33/574G01N33/577
CPCG01N33/57442G01N33/577G01N2333/70582
Inventor 史桂芝范克龙张德玺桑建彬阎锡蕴
Owner INSITUTE OF BIOPHYSICS CHINESE ACADEMY OF SCIENCES
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