Method for processing microdroplets containing samples
A droplet and sample technology, applied in biomass post-processing, laboratory containers, chemical instruments and methods, etc., can solve problems such as inability to continuously observe samples and complex problems.
Active Publication Date: 2020-11-27
ECOLE POLYTECHNIQUE +1
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- Application Information
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Problems solved by technology
[0006] However, this approach is complex, requiring a total of two separate microfluidic systems and three devices
In addition, the sample cannot be observed continuously
Method used
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Embodiment Construction
[0066] The present invention relates to a method of processing hydrogel droplets containing a sample to be tested.
[0067] The following will more particularly refer to samples in the form of cells, but of course other types of samples may be used.
[0068] The method basically comprises three steps, all carried out in a single microfluidic system, which are:
[0069] - forming droplets of a liquid aqueous solution containing one or more cells in an oil, said oil and / or said aqueous solution comprising a gelling agent,
[0070] - Trapping of liquid droplets by means of surface tension traps pre-arranged in the trapping zone, and
[0071] - gelling at least one of the oil and at least a portion of the trapped droplets.
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Abstract
The invention relates to a method for processing a droplet (14) containing a sample (16) in a microfluidic system, comprising the steps of: forming a droplet (14) of an aqueous solution containing a sample (16) in oil, the The oil and / or aqueous solution containing the sample (16) comprises a gelling agent; the droplets (14) are trapped by a surface tension trap (12) prearranged in the trapping zone (10); and at least partially gelled The oil in the trapping zone and / or at least partially gel the trapped droplets (14).
Description
technical field [0001] The present invention relates to microfluidic methods for processing samples, especially biological samples, in hydrogel droplets. The invention also relates to devices for carrying out this method and to products related to samples obtained by carrying out this method. Background technique [0002] It is known from Guo, Rotem, Heyman, and Weitz, "Droplet microfluidics for high-throughput biological assays", Lab.Chip.12 (2012), that liquid droplets (or "droplets") in microfluidic systems can be used to contain chemical reactions or biological response. In these systems, the content of the droplets can be determined by observing the fluorescence of the droplets as they pass in front of a focused laser. However, these systems cannot observe changes in the content of these droplets over time without extracting the droplets from the microfluidic device. [0003] Studies on individualized cells in droplets are also known, e.g. from Joensson, H.N. and And...
Claims
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IPC IPC(8): C12M1/00
CPCB01L3/502784B01L2200/0673B01L2200/0642B01L2400/086B01L2300/0819B01L2300/0851B01L3/502746B01L2300/0816B01L2400/088
Inventor 查尔斯·巴鲁德加布里埃尔·阿姆塞莱姆塞巴斯蒂安·撒尔塔拉斐尔·托马西
Owner ECOLE POLYTECHNIQUE