A method for stabilizing protease in laundry detergent
A technology of protease and laundry detergent, which is applied in the field of stable protease, can solve the problems of reducing enzyme activity and taking half an hour to release, and achieves the effect of high embedding rate, improved stability and good stability
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Embodiment 1
[0043] To prepare vesicles embedded with alkaline protease 2709 (909.1 mg protein / g, Xingtai Spectrum Biotechnology Co., Ltd.), the specific steps are as follows:
[0044] 1) Dissolve 5.76g of conjugated linoleic acid in 100mL of sodium dihydrogen phosphate-disodium hydrogen phosphate buffer solution (pH 8.6, 0.01mol / L) containing 0.25mmol / L calcium citrate at room temperature, conjugated linoleic acid The acid concentration is 200mmol·L -1 After mixing uniformly, add alkaline protease 2709 solution, the addition of protease is 500mg albumen / g conjugated linoleic acid (550mg protease preparation / g conjugated linoleic acid), after mixing uniformly, in 25 0 Under C, use a shaker to avoid light and oscillate for 4 hours (the shaker speed is 50rpm) to obtain a solution for embedding protease-conjugated linoleic acid vesicles, and the embedding rate is 91.2%;
[0045] 2) Put 100mL of the above solution in a 250mL stoppered cylindrical glass reactor (height-to-diameter ratio: 2:1) ...
Embodiment 2
[0052] Preparation of vesicles embedded with S. marcescens alkaline protease (930.2mg protein / g, from S. marcescens), the specific steps are as follows:
[0053] 1) Dissolve conjugated linoleic acid and calcium citrate in 0.01mol / L borax buffer (pH9.18) at room temperature and mix well. The concentration of conjugated linoleic acid is 240mmol / L, and the concentration of calcium salt is 0.15mmol / L; add viscose Saybolt alkaline protease, the amount of protease added is 560mg protein / g conjugated linoleic acid, after mixing evenly, shake it with a shaking table at 20°C for 7h, the shaking speed of the shaking table is 40rpm, Self-assembled to obtain a vesicle solution embedding protease-conjugated linoleic acid, with an embedding rate of 92.3%;
[0054] (2) Put the self-assembled protease-conjugated linoleic acid vesicle solution obtained in step (1) in the reactor, and add QTX in an amount of 0.06% of the mass of the conjugated linoleic acid in the dark, and add it to the reacto...
Embodiment 3
[0058] To prepare vesicles embedded with dispase 1398 (905.7 mg protein / g, Xingtai Spect Biotechnology Co., Ltd.), the specific steps are as follows:
[0059] (1) Dissolve conjugated linoleic acid and calcium citrate tetrahydrate in 0.01mol / L pH 8 disodium hydrogen phosphate-citric acid buffer at room temperature, the concentration of conjugated linoleic acid is 300 mmol / L, The concentration of calcium salt is 0.25 mmol / L; add neutral protease 1398 solution, the amount of protease added is 580 mg protein / g conjugated linoleic acid, mix well and shake with a shaking table at 25 °C for 5 hours, the shaking table The speed was 50 rpm, and the vesicle solution embedding protease-conjugated linoleic acid was obtained by self-assembly, and the embedding rate was 94.5%;
[0060] (2) Put the self-assembled protease-conjugated linoleic acid vesicle solution obtained in step (1) in the reactor, and add QTX in an amount of 0.08% of the mass of the conjugated linoleic acid in the dark, an...
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