Method and device for simulating intravascular fluid shear stress for detecting secretion of adherent cells

Abstract

The invention discloses a method for simulating intravascular fluid shear stress for detecting secretion of adherent cells. The method comprises the following steps: making a fluid cell by using a microfluidic chip; digesting cell trypsin reaching logarithmic growth to prepare a cell suspension, and performing cell treatment; putting the fluid cell into a culture dish, adding the cell suspension from the outlet of the fluid cell, shaking up the cell suspension in a crossing manner so as to uniformly pave the cells flatly on the bottom surface of the fluid cell, and observing a cell adherence condition under a microscope; assembling a fluid cell detection test device, and simulating the intravascular fluid shear stress for detecting the secretion condition of the adherent cells; and collecting a sample and performing a subsequent detection test. Through improvement on the material of the fluid cell, the microfluidic chip is used for making the fluid cell, so that the cells are positioned in a specific area for growth, the size of the fluid cell is reduced, the amount of the samples is reduced, the sample concentration is increased, the detection rate is increased, and increase of the detection rate helps researchers to reduce the loss and the management cost.

Description

technical field [0001] The invention relates to the field of applying microfluidic chip technology to biomedical research, in particular to an experimental method for simulating intravascular fluid shear stress and detecting secretion of adherent cells. Background technique [0002] The shear stress of the fluid in the blood vessel is not static. At the bifurcation and corner of the blood vessel in the physiological state, and at the narrow blood vessel in the pathological state, the turbulent state of low shear stress will be formed. The key factor is also a key factor in the progression of vascular disease. The fluid flows from the high-strain area to the low-strain area, and various types of cells in blood vessels are continuously exposed to low shear stress and undergo a series of reactions. The device for studying the effect of fluid shear force on cells in vitro is a loading device developed according to the principle of rheology. The basic principle is to use the flo...

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Problems solved by technology

The device for studying the effect of fluid shear force on cells in vitro is a loading device developed according to the principle of rheology. The basic principle is to use the flowing culture medium to generate shear force on the cells attached to the culture substrate. The parallel plate flow chamber is currently the A kind of fluid shear force experimental loading device commonly used at home and abroad, the sealing performance of the parallel plate flow chamber is poor, it is easy to be polluted during operation, the fluid volume demand is large, and the error of the experimental results is large

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