Application of SYCE3 in colorectal cancer diagnosis and treatment effect evaluation

A colorectal cancer, treatment effect technology, applied in the field of tumor treatment, can solve the problem of reducing curative effect, adverse consequences, etc.

Active Publication Date: 2017-09-05
新疆医科大学第四附属医院 +2
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  • Abstract
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  • Application Information

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Problems solved by technology

Traditional Chinese medicine occupies an irreplaceable position in the comprehensive treatment of advanced colorectal cancer, but the unique advantage of syndrome differentiation and treatment has a lot of subjectivity, resulting in its accuracy directly related to the clinical experience of clinicians; curative effect, and even cause serious adverse consequences

Method used

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  • Application of SYCE3 in colorectal cancer diagnosis and treatment effect evaluation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1 Differentially expressed gene screening

[0036] 1. Case collection

[0037] Patients with stage IIII-IV colorectal cancer diagnosed by pathology in the oncology department of the hospital were collected; 5 cases with internal damp-heat syndrome were selected as the experimental group, and 5 cases without internal damp-heat syndrome were selected as the control group; they only received pure Chinese medicine treatment.

[0038] 1.1 Diagnosis and staging criteria of colorectal cancer

[0039] Refer to the "Standards for the Diagnosis and Treatment of Colorectal Cancer" compiled by the Department of Medical Affairs of the Ministry of Health of the People's Republic of China in October 2010, and the first edition of the TNM staging criteria of the 2011 NCCN Tumor Clinical Practice Guidelines (Chinese Edition).

[0040] 1.2 Diagnostic criteria of various syndromes

[0041] Formulated with reference to "Diagnostics of Traditional Chinese Medicine" and "Guiding Prin...

Embodiment 2

[0057] Example 2 Verification of Differentially Expressed Genes

[0058] According to the results of Example 1, the differentially expressed SYCE3 gene was selected for large sample verification.

[0059] 1. Case collection

[0060] According to the method of Example 1, 50 cases of advanced colorectal cancer patients with intrinsic damp-heat syndrome and 50 patients with advanced colorectal cancer without intrinsic damp-heat syndrome were collected as controls.

[0061] 2. The treatment method is the same as in Example 1.

[0062] 3. Validation at the mRNA level

[0063] 3.1 Extraction of total RNA from blood

[0064] Step is with embodiment 1.

[0065] 3.2 Reverse transcription

[0066] Reverse transcription using Primescript 1 st strand cDNA synthesis kit kit, the operation steps are as follows:

[0067] (1) Add the following reaction liquid in the microcentrifuge tube, as shown in Table 1:

[0068] Table 1 Reaction liquid

[0069] Reagent dose RNA...

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Abstract

The invention discloses a tool SYCE3 gene for the diagnosis or the treatment effect evaluation of damp heat type advanced colorectal cancer patients. The content of the expression product of the SYCE3 gene in the blood of a human subject is detected to judge whether the human subject suffers from the damp heat type advanced colorectal cancer or not or evaluate the medicine intervention effect of the damp heat type advanced colorectal cancer patients. The invention also discloses a kit for the diagnosis or the treatment effect evaluation of damp heat type advanced colorectal cancer patients based on the above research accomplishment. The kit can be used for detecting blood samples, belongs to noninvasive detection, and has the advantages of sensitive and fast detection effect, and facilitation of clinic promotion and use.

Description

technical field [0001] The application belongs to the field of tumor treatment, and relates to a molecular marker for the diagnosis and treatment effect evaluation of colorectal cancer. Background technique [0002] Colorectum belongs to the category of diseases such as "intestinal accumulation", "accumulation", "mass in the abdomen", "intestinal phlegm" and "intestinal wind" in traditional Chinese medicine. Traditional Chinese medicine occupies an irreplaceable position in the comprehensive treatment of advanced colorectal cancer. However, the unique advantage of syndrome differentiation and treatment has more subjectivity, which leads to its accuracy directly related to the clinical experience of clinicians; if the syndrome differentiation is improper, it will reduce the curative effect and even cause serious adverse consequences. Therefore, the objectification of syndromes is the most important aspect of traditional Chinese medicine. An important research direction that r...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68G01N33/574
CPCC12Q1/6886C12Q2600/112C12Q2600/158G01N33/57419G01N33/57484
Inventor 何文婷节阳华张彤付晓乐帕提玛·阿布力米提陈卫东巩璇张洪亮
Owner 新疆医科大学第四附属医院
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