Large-scale preparation method of scale I-type collagen peptide applicable to cosmetics

Abstract

The invention relates to a large-scale preparation method of scale I-type collagen peptide applicable to cosmetics. The scales of marine fish or part of freshwater fish are adopted as a raw material; in pretreatment, an alkaline solution is adopted for fast degreasing, and an acid solution is adopted for fast decalcification; after that, a scale I-type collagen peptide solid obtained by the pretreatment is directly prepared into an I-type collagen peptide concentrated solution by a biological enzymolysis technology, and the extraction rate is higher than or equal to 97%; enzymatic hydrolysate is separated, purified and desalted at normal temperature by a continuous flow centrifugation collaborated membrane separation technology to obtain scale I-type collagen peptide liquid with the content of higher than or equal to 95%; finally, the scale I-type collagen peptide liquid is quickly dried by a spray drying technology to obtain scale I-type collagen peptide microspheres with a special hollow structure, and the scale I-type collagen peptide microspheres are quickly sterilized by an optical radiation technology, and are sealed and stored. According to the method provided by the invention, I-type collagen in the scales is almost completely turned into the I-type collagen peptide concentrated solution; the prepared scale I-type collagen peptide is of a microsphere shape, and the particle size is uniform and is mainly on a micron level of 1-10mu m.

Description

technical field

[0001] The invention relates to a large-scale preparation method of fish scale type I collagen peptide suitable for cosmetics, and belongs to the fields of biological extraction technology and cosmetics. Background technique

[0002] Collagen is a natural protein that widely exists in animal skin, bone, cartilage, teeth, tendons, ligaments, and blood vessels. It is an extremely important structural protein in connective tissue, which supports organs and protects the body. Strong biological activity and biological function. Collagen peptide (collagen peptide) is the hydrolyzate of collagen (collagen), which is a mixture of polypeptides or small peptides. No matter in food health care, biomedicine or cosmetics, collagen peptides have a wide range of applications and huge market value. In terms of food applications, collagen peptides can be used not only as functional foods, but also as meat improvers, and as food packaging materials—artificial casings and edi...

Examples

Embodiment 1

[0067] A large-scale preparation method of fish scale type I collagen peptide suitable for cosmetics, the steps are as follows:

[0068] (1) Pretreatment

[0069] Put the scales of American redfish (Sciaenops ocellatus) into the reaction kettle, add an alkaline solution 10 times the weight of the fish scales, stir for 30 minutes, wash with water, add an acid solution 10 times the weight of the fish scales, stir for 30 minutes, and then wash with deionized water Net, wherein the mass volume concentration of alkali solution is 3%, and the mass volume concentration of acid solution is 5%;

[0070] (2) Enzymatic extraction

[0071] Put the pretreated and washed fish scales in a reaction kettle, add deionized water of 1 times the weight of fish scales, add concentrated lye to adjust the pH to 6.5, add papain with 2% weight of fish scales, and the reaction temperature is 55 °C , and reacted for 45 minutes; after the enzymolysis was completed, the temperature was raised to 70° C., ...

Embodiment 2

[0077] A large-scale preparation method of fish scale type I collagen peptide suitable for cosmetics, the steps are as follows:

[0078] (1) Pretreatment

[0079] Pulverize the scales of bighead carp, put them into the reaction kettle, add an alkali solution twice the weight of the scales, stir for 15 minutes, wash with water, add an acid solution 10 times the weight of the scales, stir for 30 minutes, and then wash it with deionized water. Wherein the mass volume concentration of alkali solution is 4%, and the mass volume concentration of acid solution is 4%;

[0080] (2) Enzymatic extraction

[0081] Put the pretreated and washed fish scales in a reaction kettle, add deionized water, add concentrated lye to adjust the pH to 7.0, add 1.5% papain and 1.5% neutral protease by weight of the fish scales, and the reaction temperature is 60 ℃, reacted for 35 minutes, and obtained fish scale type Ⅰ collagen peptide enzymatic hydrolysis solution, the extraction rate = 99.5%.

[00...

Embodiment 3

[0087] A large-scale preparation method of fish scale type I collagen peptide suitable for cosmetics, the steps are as follows:

[0088] (1) Pretreatment

[0089] Put tilapia (Oreochromis spp) fish scales into the reaction kettle, add an alkali solution 10 times the weight of the fish scales, stir for 15 minutes, wash with water, add an acid solution 10 times the weight of the fish scales, stir for 25 minutes, and then wash with deionized water Net, wherein the mass volume concentration of alkali solution is 4%, and the mass volume concentration of acid solution is 6%;

[0090] (2) Enzymatic extraction

[0091] Put the pretreated and washed fish scales in a reaction kettle, add deionized water, add concentrated lye to adjust the pH to 6.5, add 2.5% flavor protease and 1.5% papain by weight of the fish scales, and the reaction temperature is 53°C , and reacted for 30 minutes; after the enzymolysis was completed, the temperature was raised to 75° C., and the enzyme was kept fo...

technical field

[0001] The invention relates to a large-scale preparation method of fish scale type I collagen peptide suitable for cosmetics, and belongs to the fields of biological extraction technology and cosmetics. Background technique

[0002] Collagen is a natural protein that widely exists in animal skin, bone, cartilage, teeth, tendons, ligaments, and blood vessels. It is an extremely important structural protein in connective tissue, which supports organs and protects the body. Strong biological activity and biological function. Collagen peptide (collagen peptide) is the hydrolyzate of collagen (collagen), which is a mixture of polypeptides or small peptides. No matter in food health care, biomedicine or cosmetics, collagen peptides have a wide range of applications and huge market value. In terms of food applications, collagen peptides can be used not only as functional foods, but also as meat improvers, and as food packaging materials—artificial casings and edi...

Examples

Embodiment 1

[0067] A large-scale preparation method of fish scale type I collagen peptide suitable for cosmetics, the steps are as follows:

[0068] (1) Pretreatment

[0069] Put the scales of American redfish (Sciaenops ocellatus) into the reaction kettle, add an alkaline solution 10 times the weight of the fish scales, stir for 30 minutes, wash with water, add an acid solution 10 times the weight of the fish scales, stir for 30 minutes, and then wash with deionized water Net, wherein the mass volume concentration of alkali solution is 3%, and the mass volume concentration of acid solution is 5%;

[0070] (2) Enzymatic extraction

[0071] Put the pretreated and washed fish scales in a reaction kettle, add deionized water of 1 times the weight of fish scales, add concentrated lye to adjust the pH to 6.5, add papain with 2% weight of fish scales, and the reaction temperature is 55 °C , and reacted for 45 minutes; after the enzymolysis was completed, the temperature was raised to 70° C., ...

Embodiment 2

[0077] A large-scale preparation method of fish scale type I collagen peptide suitable for cosmetics, the steps are as follows:

[0078] (1) Pretreatment

[0079] Pulverize the scales of bighead carp, put them into the reaction kettle, add an alkali solution twice the weight of the scales, stir for 15 minutes, wash with water, add an acid solution 10 times the weight of the scales, stir for 30 minutes, and then wash it with deionized water. Wherein the mass volume concentration of alkali solution is 4%, and the mass volume concentration of acid solution is 4%;

[0080] (2) Enzymatic extraction

[0081] Put the pretreated and washed fish scales in a reaction kettle, add deionized water, add concentrated lye to adjust the pH to 7.0, add 1.5% papain and 1.5% neutral protease by weight of the fish scales, and the reaction temperature is 60 ℃, reacted for 35 minutes, and obtained fish scale type Ⅰ collagen peptide enzymatic hydrolysis solution, the extraction rate = 99.5%.

[00...

Embodiment 3

[0087] A large-scale preparation method of fish scale type I collagen peptide suitable for cosmetics, the steps are as follows:

[0088] (1) Pretreatment

[0089] Put tilapia (Oreochromis spp) fish scales into the reaction kettle, add an alkali solution 10 times the weight of the fish scales, stir for 15 minutes, wash with water, add an acid solution 10 times the weight of the fish scales, stir for 25 minutes, and then wash with deionized water Net, wherein the mass volume concentration of alkali solution is 4%, and the mass volume concentration of acid solution is 6%;

[0090] (2) Enzymatic extraction

[0091] Put the pretreated and washed fish scales in a reaction kettle, add deionized water, add concentrated lye to adjust the pH to 6.5, add 2.5% flavor protease and 1.5% papain by weight of the fish scales, and the reaction temperature is 53°C , and reacted for 30 minutes; after the enzymolysis was completed, the temperature was raised to 75° C., and the enzyme was kept fo...

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