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Application of FLOT1 as human ovarian cancer biomarker

A technology of ovarian cancer, CA125, applied in the direction of measuring devices, instruments, scientific instruments, etc., to achieve the effect of improving the diagnosis rate and high incidence rate

Active Publication Date: 2017-09-15
JINSHAN HOSPITAL FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

So far, there is no report on using serum FLOT1 level as a biomarker for ovarian cancer

Method used

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  • Application of FLOT1 as human ovarian cancer biomarker
  • Application of FLOT1 as human ovarian cancer biomarker
  • Application of FLOT1 as human ovarian cancer biomarker

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1 Animal experiments screen out potential ovarian cancer biomarkers

[0023] 1. Collect OVCAR-3 cells, wash them twice with PBS, and suspend them in serum-free RPMI-1640 medium to a concentration of 5*10 7 / ml.

[0024] 2. BALB-nude nude mice were randomly divided into Blank and OVCAR-3 subcutaneous injection groups, 10 mice / group. SPF level room breeding, room temperature 26-28 ℃, relative humidity 40%-60%, 10 hours of light, 14 hours of light-dark cycle without light. OVCAR-3 cells 5*10 6 / 100μl, subcutaneous injection in armpit. When the volume of subcutaneous tumor reaches 100mm 3 The mice were killed with 10% chloral hydrate, and the serum was collected.

[0025] see results figure 1 , indicating that the ovarian cancer xenograft mouse model was successfully established.

[0026] 3. The expression level of mouse serum FLOT1 was detected by ELISA, and CA125 was used as the control.

[0027] ELISA kits were purchased from Shanghai Enzyme Biotechnology...

Embodiment 2

[0045] Example 2 Comparison and combined diagnosis of FLOT1 and CA125 in the diagnosis of human ovarian cancer

[0046]1. FLOT1 and CA125 are highly expressed in human ovarian cancer serum. Serum from 18 cases of ovarian cancer patients and 18 cases of normal controls. The serum of ovarian cancer patients was the serum before operation and without radiotherapy and chemotherapy, and the serum of normal control was the serum of healthy people, and the age was similar to the median age of ovarian patients, and there was no statistical significance in the age of the two groups. The expression level of FLOT1 in human serum was detected by ELISA, and CA125 was used as the control (**, P<0.01).

[0047] see results image 3 , indicating that compared with healthy people, ovarian cancer patients have significantly increased serum FLOT1 and CA125, and FLOT1 has high accuracy as a diagnostic marker for ovarian cancer.

[0048] 2. Use IBM SPSS Statistics 21 software to make ROC curve....

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Abstract

The invention relates to an application of FLOT1 as a human ovarian cancer biomarker. It is confirmed that the ovarian cancer can be diagnosed through detecting the FLOT1 level of human serum, the cutoff value of the FLOT1 is 1233 pg / ml, the sensitivity is 0.722, the 1-specificity is 0.278, and the AUC value is 0.787, so the FLOT1 can be used as an effective biomarker of the ovarian cancer, and the diagnostic value is high; and it is also confirmed that the diagnosis rate of the ovarian cancer is improved by 24% by combining the FLOT1 with CA125 diagnosis. It is suggested that a reagent used for detecting the FLOT1 level of the serum can be used to prepare reagents or kits for diagnosing the ovarian cancer, and kits containing the serum FLOT1 and CA125 level detection reagent can significantly improve the diagnosis rate of the ovarian cancer. The application provides a new way for the accurate diagnosis of the ovarian cancer.

Description

technical field [0001] The present invention relates to the field of tumor diagnosis, in particular, relates to the application of FLOT1 as a biomarker of ovarian cancer. Background technique [0002] Ovarian cancer is one of the common malignant tumors of female reproductive organs, and its incidence ranks third after cervical cancer and uterine body cancer. Due to the complicated embryonic development, tissue anatomy and endocrine function of the ovary, the early symptoms are not typical. Therefore, exploring biomarkers and therapeutic targets of ovarian cancer is of great significance for the diagnosis and treatment of ovarian cancer. Currently, CA125 is commonly used as a clinical biomarker for the diagnosis of ovarian cancer. The literature shows that CA125 is only expressed in about 70% of ovarian cancer patients (Gynecol Oncol, 2007.104(3): p.508-15.), and not in It can be detected in the blood of all ovarian cancer patients (J Clin Oncol, 2009.27(15_suppl): p.e1657...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/574
CPCG01N33/57449G01N33/57484
Inventor 许国雄张凌云
Owner JINSHAN HOSPITAL FUDAN UNIV
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