Ultrasonic diagnosis target reagent for liver cancer and preparation method thereof

Abstract

The invention discloses an ultrasonic diagnosis target reagent for liver cancer. The reagent is an Endoglin target microbubble, the target microbubble comprises a lipid casing and a gas inner core, and an Endoglin monoclonal antibody is connected to the outer surface of the lipid casing. The lipid casing is prepared from dipalmitoyl phosphoethanolamine (DPPE), distearoyl phosphoethanolamine (DSPE) and polyethylene glycol 1500 which are mixed, and the gas inner core is biological inert gas and further optimized as perfluoropropane gas (C3F8). According to the designed ultrasonic diagnosis target reagent disclosed by the invention, the target microbubble can penetrate through tumor vessels through intravenous injection to enter tumor tissue gaps and be specifically combined with adult vascular endothelial cells and mesenchymal cells; thus, the microbubble can effectively enrich at the tumor position, retention of the microbubble is prolonged, lesion tissues and peripheral normal tissues can be clearly distinguished through gathering imaging, and a non-linear harmonic imaging technology is utilized to achieve target molecular imaging of the liver cancer.

Description

technical field [0001] The invention relates to a targeting reagent for ultrasound diagnosis, in particular to a targeting reagent for ultrasound diagnosis of liver cancer and a preparation method thereof. Background technique [0002] Liver cancer is an aggressive cancer that causes high mortality in Asia, and early diagnosis and treatment are important to improve the survival chances of liver cancer patients. Ultrasound examination is an important method for clinical diagnosis of tumors due to its advantages of non-invasive and radiation-free, convenient operation, low cost, and low requirements for equipment and space. However, due to the limitations of ultrasonographic diagnosis itself, it is difficult to detect small liver cancer tissues. [0003] With the emergence of various new ultrasound contrast agents and the continuous development of ultrasound imaging technology, microbubble contrast agents (such as Sonovue contrast agent produced by Braco Company in China) have...

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Problems solved by technology

[0005] In the prior art, the method of linking specific antibodies or ligands to the surface of microbubbles usually adopts biotinylation of specific antibodies or ligands, and then adding avidin, and then through the linking effect of avidin and biotin to make Specific antibodies or ligands are connected to microbubbles. However, there are certain problems in the above-mentioned connection. Because the biotinylation of antibodies or ligands may affect their biological activity and be affected by the shear force of blood flow, targeting ultrasound contrast agents It cannot be tightly adsorbed to the target tissue, which reduces the targeting function, which is especially evident in the contrast agent of liver cancer

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