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A kind of aflatoxin b1 immune rapid detection device and detection method

A technology of aflatoxin and detection device, which is applied in the field of immunoassay, can solve the problems of long detection time, cumbersome separation and washing steps, etc., and achieve the effect of ensuring safety

Active Publication Date: 2019-06-28
NANJING YITE BIOTECH
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  • Abstract
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  • Claims
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Problems solved by technology

However, the ELISA method still requires cumbersome separation and washing steps, making the detection time still longer. The development of simpler and faster detection methods is the current research hotspot and difficulty in immunoassay detection.

Method used

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  • A kind of aflatoxin b1 immune rapid detection device and detection method
  • A kind of aflatoxin b1 immune rapid detection device and detection method
  • A kind of aflatoxin b1 immune rapid detection device and detection method

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Effect test

Embodiment

[0037] Example: Aflatoxin B 1 Design and application of integrated immunological rapid detection device

[0038] 1 detection device

[0039] Such as figure 2 , 3 As shown, the integrated detection device was fabricated using polydimethylsiloxane (PDMS) as the material. The mold is made of bronze, polished and then chrome plated. The detection device has three functional areas, which are the sample chamber, the washing chamber and the detection chamber, with a size of 45×10×8mm 3 , the effective volume is 300 μL / chamber. Each chamber has a cover plate for sample addition and sampling; the chambers are connected by a 1.5mm wide hole as the passage of the compound under the magnetic field; the chambers are connected by 1×0.6×7mm 3 The special glass inserts realize sealing and isolation, so that each functional area does not interfere with each other. Pour the PDMS prepolymer into the mold, let it stand at 70°C for 60 minutes, and cool to obtain the detection device.

[0...

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Abstract

The invention provides a fast immune detecting device and a detecting method for aflatoxin B1. The fast immune detecting device for the aflatoxin B1 comprises a sample chamber, a washing chamber and a detecting chamber which are used as three functional zones; one cover plate is arranged on each of the sample chamber, the washing chamber and the detecting chamber and is used for adding samples and taking the samples; the sample chamber is communicated with the washing chamber by a pore channel; the washing chamber is communicated with the detecting chamber by a pore channel; a glass plug board is arranged at each pore channel, so isolation between every two functional zones is realized, and mutual intervention is avoided. The detecting method comprises the following steps: preparing a functionalized probe; carrying out homogeneous immune response and magnetic separation; developing and reading. An integrated fast immune detecting device and a detecting strategy provided by the invention can be applied to fast detection of the aflatoxin B1, and have guidance and reference value for a fast immune detecting technology of other small molecule toxic or harmful substances.

Description

technical field [0001] The present invention relates to a kind of aflatoxin B based on homogeneous immune reaction and magnetic separation technology 1 An integrated rapid detection device and a detection strategy belong to the technical field of immune detection. Background technique [0002] With the development of social economy and the improvement of science and technology, human beings pay more and more attention to health and safety. At present, many consumers are in a sub-healthy state, which is directly or indirectly related to the safety of food and the environment. What's more serious is that some food and environmental toxic and harmful substances may also induce malignant diseases such as blood diseases and cancers, posing a threat to consumers' lives. [0003] Aflatoxin B 1 It is an extremely toxic secondary metabolite mainly produced by Aspergillus flavus and Aspergillus parasiticus, mainly present in grain, oil, animal and plant foods, peanut and corn are t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/577
CPCG01N33/577
Inventor 李明张媛媛崔银杜道林
Owner NANJING YITE BIOTECH
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