Preparation method of ferment type bitter gourd solid beverage
A solid beverage, bitter gourd technology, applied in the direction of bacteria, sugary food ingredients, food ingredients used in food preparation, etc., can solve the problems of affecting nutritional value and health care efficacy, complex methods, reducing bitterness, etc., to achieve excellent taste. , the effect of reducing bitterness and improving solubility
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Embodiment 1
[0030] Bacillus natto suitable for preparing food is isolated from commercially available food containing live Bacillus natto, and is preserved on a potato solid slant after purification and biochemical identification. Lactobacillus plantarum is isolated from yogurt containing Lactobacillus plantarum, and preserved on THB solid slant after microscopic inspection and purification.
[0031] Pick Bacillus natto from the solid slant and inoculate it in 10mL PDA liquid medium; pick Lactobacillus plantarum from the solid slant and inoculate it in 10mL THB liquid medium. Cultivate at 32°C for 12 hours to prepare primary seed solution.
[0032] Inoculate 10 mL of primary seed liquid of Bacillus natto and 10 mL of primary seed liquid of Lactobacillus plantarum into 350 mL of PDA liquid medium and 350 mL of THB liquid medium, respectively, and incubate at 30°C for 12 hours to prepare secondary seed liquid.
[0033] Pretreatment and homogenization of bitter gourd: choose fresh seven-rip...
Embodiment 2
[0042] Bacillus natto CICC 10262 was purchased from China Industrial Microbiology Culture Collection Center (CICC) as a fermentation strain of Bacillus natto.
[0043] The Lactobacillus plantarum reference strain ATCC 14917 preserved in the inventor's laboratory was used as the Lactobacillus plantarum fermentation strain.
[0044]Pick Bacillus natto CICC 10262 from the solid slant and inoculate it in 10 mL PDA liquid medium; pick Lactobacillus plantarum ATCC 14917 from the solid slant and inoculate it in the mixed liquid medium containing 5 mL THB+5 mL skim milk. Cultivate at 32°C for 12 hours to prepare primary seed solution.
[0045] Inoculate 10 mL of primary seed liquid of Bacillus natto and 10 mL of primary seed liquid of Lactobacillus plantarum into 350 mL of PDA liquid medium and 350 mL of THB liquid medium, respectively, and incubate at 30°C for 12 hours to prepare secondary seed liquid.
[0046] Pretreatment and homogenization of bitter gourd: choose fresh seven-ripe...
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