Internal reference gene for melon fruit genet PCR expression analysis, and stability verification method thereof
An internal reference gene and stability technology, applied in the field of plant genetic engineering, can solve the problems of poor stability and poor accuracy of melon gene expression analysis, and achieve the effects of wide application range, wide applicability and high expression stability
Inactive Publication Date: 2017-10-03
武汉市农业科学技术研究院作物科学研究所
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The present invention discloses an internal reference gene for melon fruit genet PCR expression analysis, and a stability verification method for the internal reference gene. According to the present invention, the expression level and the stability of the gene are compared at the whole genome level by using melon transcriptome data so as to select eight stably-expressing genes, two traditional internal reference genes commonly used in melon are selected as controls, the expression stability of the 10 genes in two melon variety fruits at different development stages are compared, and it is determined that the expression stability of the MELO3C007745T1 gene is high highest through verification with genes such as Cmain2 and CmSPS1m, wherein the MELO3C007745T1 gene is the optimal internal reference gene for the real-time fluorescence quantitative PCR analysis in the melon fruit gene expression.
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