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Short-chain peptide derived from tumor antigen PRAME

A complex, amino acid technology, used in tumor-specific antigens, tumor rejection antigen precursors, anti-tumor drugs, etc.

Active Publication Date: 2017-10-20
XLIFESC LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

After the tumor tissue is processed, the peptide fragments presented on the surface of the tumor cells can be directly identified by mass spectrometry. Although this is a complicated process, the results obtained in this way are very reliable

Method used

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  • Short-chain peptide derived from tumor antigen PRAME
  • Short-chain peptide derived from tumor antigen PRAME
  • Short-chain peptide derived from tumor antigen PRAME

Examples

Experimental program
Comparison scheme
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preparation example Construction

[0084] The preparation methods of the above TCR and antibody are known to those skilled in the art, including but not limited to, expressing and purifying from Escherichia coli cells or insect cells.

[0085] In another aspect, the present invention further provides the use of the peptides, pMHC complexes, nucleic acid molecules, vectors, cells and binding molecules of the present invention in pharmaceuticals. The peptide, pMHC complex, nucleic acid, vector, cell or binding molecule can be used to treat or prevent cancer, preferably melanoma, bladder cancer, liver cancer, epidermoid cancer, non-small cell lung cancer and squamous cell carcinoma, etc.

[0086] The present invention also provides a pharmaceutical composition, which comprises the peptide of the present invention, the pMHC complex, the nucleic acid molecule of the present invention, the cell of the present invention or the binding molecule of the present invention, and a pharmaceutically acceptable carrier. The ph...

Embodiment 1

[0093] Example 1 Identification of polypeptides derived from PRAME antigens by mass spectrometry

[0094] Before the identification by mass spectrometry, the present invention further verifies that the PRAME antigen is expressed in various tumor cells. Specifically, a digital single-molecule multiplex gene expression profiling system is used for detection (nanostring). The results showed that PRAME antigen was expressed in tumor cells such as melanoma, adenocarcinoma, non-small cell lung cancer, hepatocellular carcinoma, myeloma, osteosarcoma, renal cell carcinoma, and lymphoma.

[0095]The HLA-short peptide complex was purified using commercial antibody W6 / 32. Specifically, the tumor cells were lysed with a buffer solution containing the non-ionic surfactant Triton X-100 (1% v / v), 1 ml of the lysate was added to 2*10^7 cells, and the tumor cells were incubated at 4° C. for 1 hour by rolling. The cell debris was removed by centrifugation, and the supernatant was first incuba...

Embodiment 2

[0102] The preparation of embodiment 2 soluble pMHC complexes

[0103] The heavy chain and light chain (β2m) of type I HLA-A*1101 molecules were expressed in E. coli in the form of inclusion bodies. It should be noted that the heavy chain of the HLA-A*1101 molecule used in this example does not contain its transmembrane and cytoplasmic regions in order to obtain soluble pMHC complexes. In addition, in order to facilitate the subsequent biotinylation of the soluble pMHC complex, a biotinylation tag can be added to the C-terminus of the heavy chain. The specific process of preparing the soluble pMHC complex of the present invention is as follows:

[0104] a.Purification

[0105] Collect 100ml of E.coli bacteria liquid induced to express heavy chain or light chain, centrifuge at 8000g at 4°C for 10min, wash the bacteria once with 10ml PBS, then resuspend the bacteria with 5ml BugBuster Master Mix Extraction Reagents (Merck) vigorously shake, and place in Rotate and incubate at...

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Abstract

The invention relates to a newly discovered short peptide derived from a tumor antigen PRAME, a complex formed by the short-chain peptide and aMHC molecules, and application of the short-chain peptide and complex. At the same time, the invention also relates to molecules combined with the short-chain peptide or the complex above, and application of the molecules.

Description

technical field [0001] The present invention relates to a newly discovered short peptide derived from tumor antigen PRAME, a complex formed between the short peptide and MHC molecules and the use of the short peptide and the complex. At the same time, the present invention also relates to molecules combined with the above-mentioned short peptides or complexes, and uses of these molecules. Background technique [0002] It is well known that in many pathological conditions, such as infection, cancer, autoimmune diseases, etc., there will be inappropriate expression of some specific molecules. These molecules thus become "markers" of a pathological or abnormal state. These molecules can not only be used as markers for disease diagnosis, but also be used to produce diagnostic reagents and / or therapeutic agents. For example, markers of cancer are used to generate specific antibodies. In addition, these molecules can also effectively stimulate the specific immune response of cy...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/47A61K38/10A61K39/00A61P35/00
CPCC07K14/4748A61K39/00A61K38/00
Inventor 李懿余晓红龚海平刘秋萍陈琳吴万里
Owner XLIFESC LTD
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