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Identification method of vitis amurensis germplasm resource and application

An identification method, the technology of Vitis vinifera, applied in the identification field of Vitis vinifera germplasm resources, can solve the problems of seedlings with the same name and different objects, affecting the development of the Vitis vinifera industry, the same object with different names, etc., and achieve the effect of improving identification efficiency

Active Publication Date: 2017-10-20
INST OF SPECIAL ANIMAL & PLANT SCI OF CAAS
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  • Abstract
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Problems solved by technology

[0004] Vitis vinifera is rich in resources. At present, resource identification is mainly based on morphological markers. This method is simple but is easily affected by seasonal changes, cultivation management methods, environmental factors and subjective judgment factors. Phenomenon that seriously affects the development of the mountain grape industry

Method used

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  • Identification method of vitis amurensis germplasm resource and application
  • Identification method of vitis amurensis germplasm resource and application
  • Identification method of vitis amurensis germplasm resource and application

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Embodiment

[0054] 1 Materials and methods

[0055] 1.1 Materials

[0056] In the present invention, 11 resource varieties of Vitis vinifera germplasm were selected and three replicates were set up (Table 1), including 33 samples of estrogenous flowers, hermaphroditic flowers, tetraploids and male flowers with good traits. The test materials were all collected from the National Fruit Tree Germplasm Zuojia Mountain Grape Garden of the Institute of Special Products, Chinese Academy of Agricultural Sciences, collected according to the planting map of the mountain grape garden, and the collected fresh leaves were marked and numbered, and then quickly dried in color-changing silica gel.

[0057] Table 1 Variety information of mountain grape resources

[0058]

[0059] 1.2 Method

[0060] 1.2.1 Total DNA extraction, PCR amplification and sequencing of Vitis vinifera

[0061] Weigh 20 mg of the leaves of Vitis vinifera resources dried on silica gel, add liquid nitrogen to fully grind, use ...

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Abstract

The invention relates to the technical field of molecular identification, and in particular relates to an identification method of a vitis amurensis germplasm resource and application, and the method comprises the following steps: amplification of psbA-TrnH and / or ITS2 sequence fragment of a to-be-tested sample and sequencing; and sequence alignment according to psbA-TrnH and / or ITS2 barcode sequences of different known vitis amurensis species to determine the vitis amurensis specie of the to-be-tested sample. According to the identification method of the vitis amurensis germplasm resource, the psbA-TrnH sequence is combined with the ITS2 sequence, the psbA-TrnH sequence is used as a DNA barcode sequence of the vitis amurensis germplasm resource, the ITS2 sequence is used as a complementary sequence of the psbA-TrnH sequence, the identification efficiency of the vitis amurensis germplasm resource is improved, and the identification method can be used for identification of intraspecific and interspecific resources of vitis amurensis.

Description

technical field [0001] The invention relates to the technical field of molecular identification, in particular to an identification method of grape germplasm resources and its application. Background technique [0002] Vitis amurensis belongs to the East Asian population, is a deciduous vine of the genus Vitis in the grape family, and is the most cold-resistant species in the genus Vitis. Branches and tendrils can withstand low temperatures of -45°C, and roots can withstand low temperatures of -16°C. They are native to Northeast China, North my country, North Korea, and the Far East of the former Soviet Union. Wild Vitis vine resources are extremely rich in Changbai Mountains and Xiaoxing'an Mountains in my country, and it is one of the most successful domesticated and cultivated wild fruit trees in my country. The berries of Vitis vinifera are rich in nutritional value and are high-quality raw materials for brewing high-grade wines. Pure Vitis vinifera also has strong resi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
CPCC12Q1/6895
Inventor 艾军李昌禹宋慧芳刘海双刘迎雪杨义明范书田许培磊石广丽赵滢
Owner INST OF SPECIAL ANIMAL & PLANT SCI OF CAAS
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