Method for researching growth of sleeve-fish individuals based on inner shells and front carapaces
A technology of inner shell, fish, applied in the direction of application, fish farming, animal husbandry, etc.
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[0046] A method for studying the individual growth of the kite squid by using the growth pattern of the inner shell forecarp, comprising the following steps:
[0047] (1) Extraction: Use a scalpel, scissors and other similar tools to carefully extract the inner shell of the squid to prevent the inner shell from breaking and breaking;
[0048] (2) Cleaning: Clean the surface of the inner shell with clean water;
[0049] (3) Preservation: store the cleaned inner shell in a refrigerator at -20°C;
[0050] (4) Soaking: Soak in distilled water for 5 minutes to restore the inner shell to its natural state;
[0051] (5) Staining solution configuration: add 0.1g alizarin red powder into 100ml distilled water to make 0.1% alizarin red dyeing solution;
[0052] (6) Dyeing: the inner shell is placed in 0.1% alizarin solution and soaked for about 24 hours;
[0053] (7) Rinse: Rinse the dyed inner shell sample with running water for about 12 hours, until the inner shell does not flow ou...
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