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Chimeric antigen receptor modified dendritic cells for treating glioma and preparation method thereof

A chimeric antigen receptor and dendritic cell technology, applied in the field of biomedicine, can solve the problems of affecting the curative effect of cellular immunity, limited curative effect, and easy recurrence

Active Publication Date: 2019-12-17
SHILI BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, CAR-T will produce certain off-target toxicity, which will affect its cellular immune efficacy; more importantly, due to the heterogeneity of tumors, there are often multiple tumor antigens in the same tumor, so CAR-T targeting a single tumor antigen Limited curative effect and prone to relapse

Method used

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  • Chimeric antigen receptor modified dendritic cells for treating glioma and preparation method thereof
  • Chimeric antigen receptor modified dendritic cells for treating glioma and preparation method thereof
  • Chimeric antigen receptor modified dendritic cells for treating glioma and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0045] 1. Determination of a EGFRvIIICAR gene sequence

[0046] 1.1 Obtain human CD8α signal peptide gene (SEQ ID NO.3), human CD8α hinge region gene (SEQ ID NO. .7), human CD40 transmembrane region (SEQ ID NO.8) and human CD40 signal domain gene (SEQ ID NO.9) sequence. Human CD8α signal peptide gene (SEQ ID NO.3), EGFRvIII scFv light chain gene (SEQ ID NO.4), linker region (Gly4-Ser) 3 (SEQ ID NO.5), EGFRvIII scFv heavy chain gene (SEQ ID NO.6), human CD8α hinge region gene (SEQ ID NO.7), human CD40 transmembrane region (SEQ ID NO.8) and human CD40 signal domain gene (SEQ ID NO.9) sequences were connected to form the final complete EGFRvIIICAR gene sequence (SEQ ID NO.2) information.

[0047] 2. Construction of EGFRvIIICAR expression plasmid

[0048] 2.1 Whole gene synthesis:

[0049] The complete EGFRvIIICAR sequence (SEQ ID NO.2) was synthesized by Shanghai Sangon Biotechnology Co., Ltd., and an Xba I restriction site was added to its 5' end, and an EcoR I restriction s...

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Abstract

The invention relates to a chimeric antigen receptor, a chimeric antigen receptor modified dendritic cell for treating brain glioma and preparation method thereof, application of the dendritic cell in treating the brain glioma and application of the dendritic cell in preparing medicine for treating the brain glioma.

Description

technical field [0001] The invention belongs to the field of biomedicine, and in particular relates to a chimeric antigen receptor (Chimeric Antigen Receptor, CAR) and a dendritic cell (CAR-DC) modified by the chimeric antigen receptor, and a preparation method thereof , and the use of the dendritic cells in the preparation of medicines for treating glioma. Background technique [0002] Dendritic cells are a type of white blood cells that are different in shape and function from granulocytes, macrophages, and lymphocytes. Their cell membranes protrude outward to form membranous dendritic protrusions similar to nerve cell axons, named dendrites. Dendritic cells (DC). It is the professional antigen-presenting cell with the strongest function in the body, which can efficiently absorb, process and present antigens. DC binds to the captured and processed tumor antigens through the MHC molecules on the surface to form peptide-MHC molecular complexes and presents To T cells, ther...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K19/00C12N15/62C12N15/867C12N5/10A61K35/15A61P35/00
CPCA61K35/15C07K14/7051C07K16/2863C07K2317/622C07K2319/02C07K2319/03C07K2319/33C07K2319/74C12N15/86C12N2740/15043
Inventor 樊克兴高同同
Owner SHILI BIOTECH CO LTD