Nucleic acid for coding chimeric antigen receptor protein and T lymphocyte for expression of chimeric antigen receptor protein

A technology of chimeric antigen receptors and nucleic acids, applied in the direction of receptors/cell surface antigens/cell surface determinants, genetically modified cells, cells modified by introducing foreign genetic material, etc., can solve tissue damage, off-target cytotoxicity , reducing the activation threshold of effector cells, etc.
CN104087607AInactive Publication Date: 2014-10-08CRAGE MEDICAL CO LTD
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
CRAGE MEDICAL CO LTD
Publication Date
2014-10-08
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a nucleic acid for coding a chimeric antigen receptor protein expressed on the surface of the human T lymphocyte. The chimeric antigen receptor protein comprises an extracellular binding domain, a transmembrane domain and an intracellular signal domain, and the extracellular binding domain, the transmembrane domain and the intracellular signal domain are orderly connected. The extracellular binding domain comprises a single-chain antibody scFv (epidermal growth factor receptor EGFR) for specific recognition of 287th-302th amino acid epitopes of EGFR.
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Description

technical field

[0001] The present invention relates to the field of tumor cell therapy, more specifically, to the field of transgenic T lymphocyte therapy for epithelial-derived tumors expressing EGFRvIII or highly expressing EGFR. Background technique

[0002] Epidermal Growth Factor Receptor (EGFR) is a transmembrane glycoprotein with a molecular weight of 170KD. It is the expression product of the proto-oncogene C-erbB-1 (HER-1), and is widely distributed in various tissues of the human body. On the cell membrane [Alan Wells. Molecules in focus EGF receptor. Int J Biochem Cell Biol, 1999, 31: 637-643.]. It is overexpressed and (or ) mutations are closely related to tumor development, malignant transformation, metastasis and prognosis [Jose B. Why the epidermal growth factor receptor? The rational for cancer therapy [J]. Oncologist, 2002, 7(4): 2-8.]. Therefore, EGFR is an important target for tumor therapy. In addition, studies have shown that the EGFR287-302 epitope...

Claims

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