Application of psoralea corylifolia in preparation of medicine for treating children fatty liver
A technology of psoraleae and fatty liver, which is applied in the field of application of psoralen in the preparation of medicines for treating fatty liver in children, can solve problems such as no report on the therapeutic effect and mechanism of fatty liver in children, and achieves improvement of fatty liver, The effect of reducing inflammatory cell infiltration and hepatocyte steatosis
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[0050] Example 1 Effect of Psoralen on OGTT and HOMA-IR in juvenile fatty liver mice
[0051] 1. Oral glucose tolerance test (OGTT) method
[0052] First, the OGTT experiment was performed on the mice 3 days before sacrifice. The mice were fasted for 12 hours, and blood samples were collected by cutting the tail and taking blood. The fasting blood glucose (FBG) value was measured with a Roche blood glucose meter / excellent type. After that, the mice were given 50% glucose 2g / kg by gavage, and then the tail was cut again to take blood. The instrument measures blood glucose (PG-1h, PG-2h) values 1h and 2h after intragastric administration.
[0053] 2. Method of calculating insulin resistance index (HOMA-IR)
[0054] 1) Fasting blood glucose (FBG) was measured by glucose oxidase method, and fasting insulin (FINS) was measured by ELISA method. Calculate HOMA-IR according to the formula: FBG(mmol / L)×FINS(mIU / L) / 22.5.
[0055] 3. Results
[0056] As shown in Figure 1: Compared with the nor...
Example Embodiment
[0062] Example 2 Effect of psoralen on blood lipids in juvenile fatty liver mice
[0063] 1. Method
[0064] The mice were fasted for 12 hours before being sacrificed, and then the eyeballs were removed to collect blood samples, the blood volume was 2-3ml, centrifuged at 3000r / min for 30min, the serum was separated, and the blood TC value, TG value and HDL-C value were determined by enzymatic method.
[0065] As shown in Table 2: Compared with the normal group, the model mice developed dyslipidemia, and the serum TG value, TC value and LDL-C value increased significantly (p <0.01), and the serum HDL-C level was significantly reduced (p <0.01); Compared with the model group, the individual blood lipid levels of each dose group of psoralen and the vitamin E group were improved to varying degrees (p <0.01, p <0.05), among which the high-dose psoralen group improved the most significantly (p <0.01), the difference was statistically significant in the lower dose group (p <0.01, p <0.05).
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Example Embodiment
[0070] Example 3 Effect of psoralen on liver morphology of juvenile fatty liver mice
[0071] 1. Method
[0072] a. Material handling:
[0073] The mice were fasted for 12 hours before being sacrificed, and liver tissues were taken. The right middle lobe, upper right lobe and lower right lobe of the liver were fixed with 4% paraformaldehyde, embedded in paraffin, and used for hematoxylin-eosin (HE) detection as backup;
[0074] b. Hematoxylin-Eosin (HE) staining
[0075] Take paraffin sections, deparaffinize with xylene, hydrate with gradient ethanol, rinse with deionized water, stain with hematoxylin for 1-5min, rinse with deionized water for 2min, differentiate with 1% hydrochloric acid and alcohol for 15sec, rinse with deionized water for 1min, and pan with 1% ammonia water Blue 30sec, deionized water rinse for 1min, eosin staining for 30sec, deionized water rinse for 30sec, gradient ethanol dehydration, xylene transparent, dry, neutral gum mount, and observe in an optical microscop...
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