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Application of psoralea corylifolia in preparation of medicine for treating children fatty liver

A technology of psoraleae and fatty liver, which is applied in the field of application of psoralen in the preparation of medicines for treating fatty liver in children, can solve problems such as no report on the therapeutic effect and mechanism of fatty liver in children, and achieves improvement of fatty liver, The effect of reducing inflammatory cell infiltration and hepatocyte steatosis

Inactive Publication Date: 2017-11-17
周俪姗
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In addition to the effects of anti-tumor and improving osteoporosis, however, the therapeutic effect and mechanism of PC on fatty liver in children have not been reported.

Method used

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  • Application of psoralea corylifolia in preparation of medicine for treating children fatty liver
  • Application of psoralea corylifolia in preparation of medicine for treating children fatty liver
  • Application of psoralea corylifolia in preparation of medicine for treating children fatty liver

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Example 1 Effect of psoralen on OGTT and HOMA-IR in young fatty liver mice

[0051] 1. Oral Glucose Tolerance Test (OGTT) Method

[0052] Firstly, the OGTT experiment was performed on the mice 3 days before sacrifice. The mice were fasted for 12 hours, and the blood sample was collected by cutting the tail, and the fasting blood glucose (FBG) value was detected with the Roche blood glucose meter / excellent type, and then 50% glucose 2g / kg was administered into the stomach, and then the tail was cut again to obtain blood, the blood glucose The blood glucose (PG-1h, PG-2h) values ​​were measured 1h and 2h after intragastric administration respectively.

[0053] 2. Calculation method of insulin resistance index (HOMA-IR)

[0054] 1) Glucose oxidase method was used to measure fasting blood glucose (FBG), and ELISA method was used to measure fasting insulin (FINS). HOMA-IR was calculated according to the formula: FBG(mmol / L)×FINS(mIU / L) / 22.5.

[0055] 3. Results

[0056]...

Embodiment 2

[0062] Example 2 Effect of psoralen on blood lipids in young mice with fatty liver

[0063] 1. Method

[0064] The mice were fasted for 12 hours before being sacrificed, and then the blood samples were collected by enucleating the eyeballs. The blood collection volume was 2-3ml, centrifuged at 3000r / min for 30min, the serum was separated, and the blood TC value, TG value and HDL-C value were measured by enzymatic method.

[0065] As shown in Table 2: Compared with the normal group, the model mice had dyslipidemia, and the serum TG value, TC value and LDL-C value were all significantly increased (p<0.01), and the serum HDL-C level was significantly reduced (p <0.01); compared with the model group, the levels of individual blood lipids in each psoralea dose group and vitamin E group were improved to varying degrees (p<0.01, p<0.05), and the improvement in the high-dose psoralen group was the most obvious ( p<0.01), the difference was statistically significant in the lower dose ...

Embodiment 3

[0070] Example 3 Effect of psoraleae on liver morphology of young fatty liver mice

[0071] 1. Method

[0072] a. Material handling:

[0073] The mice were fasted for 12 hours before being sacrificed, and the liver tissue was taken, and the right middle lobe, right upper lobe and right lower lobe of the liver were fixed with 4% paraformaldehyde, embedded in paraffin, and used for hematoxylin-eosin (HE) detection for future use;

[0074] b. Hematoxylin-eosin (HE) staining

[0075] Paraffin sections were taken, dewaxed with xylene, hydrated with graded ethanol, rinsed with deionized water, stained with hematoxylin for 1-5 minutes, rinsed with deionized water for 2 minutes, differentiated with 1% hydrochloric acid alcohol for 15 seconds, rinsed with deionized water for 1 minute, flooded with 1% ammonia water Blue for 30 sec, rinse with deionized water for 1 min, stain with eosin for 30 sec, rinse with deionized water for 30 sec, dehydrate with gradient ethanol, clear with xylen...

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Abstract

The invention relates to the technical field of medicine, and concretely relates to an application of psoralea corylifolia in preparation of a medicine for treating children fatty liver. The psoralea corylifolia can improve fatty liver of young mice, wherein the curative effect presents in a dose-dependent manner; can improve early hepatic fibrosis of young mice with fatty liver, wherein the curative effect presents in the dose-dependent manner; and can inhibit the NF(nuclear factor)-kB inflammatory pathway activation, wherein the curative effect presents in the dose-dependent manner. By establishing a young mice NAFLD model, the psoralea corylifolia has the effect for inhibiting the hepatic tissue NF-kB activity, and has prevention and treatment effects for children fatty liver. The psoralea corylifolia can be combined with other compounds for preparing a medicinal preparation for treating children fatty liver.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to the application of psoraleae in the preparation of medicines for treating fatty liver in children. Background technique [0002] Nonalcoholic fatty liver disease (NAFLD) presents a trend of globalization and younger age prevalence, and is the primary cause of children seeking gastroenterology or liver disease specialists. Obesity is closely related to fatty liver. The prevalence of childhood obesity in my country is an objective fact. The prevalence of NAFLD in obese children is as high as 68.2%. NAFLD originating in childhood is the main reserve group for adult liver cirrhosis and metabolic syndrome-related components. With the process of urbanization in my country, the rise of middle-class families and the full opening of the "two-child" policy, NAFLD has spread rapidly and has replaced chronic viral hepatitis as a major problem threatening the health of children in my countr...

Claims

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Application Information

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IPC IPC(8): A61K36/487A61K9/20A61K9/48A61P1/16A01K67/02
CPCA01K67/02A61K9/1652A61K9/2059A61K36/48A61K36/487A61K2300/00
Inventor 周俪姗
Owner 周俪姗
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