Cosmetic composition for skin regeneration and pharmaceutical composition for wound treatment, containing sinapic acid, which is marker component of cynanchum atratum extracts, or cynanchum atratum extracts containing same

A cosmetic composition and skin regeneration technology, applied in the directions of medical preparations, cosmetics, and drug combinations containing active ingredients, can solve problems such as limitation, irritation of the skin, side effects of skin regeneration ability, etc., and achieve excellent wound treatment effect, excellent cell effect of regeneration

Active Publication Date: 2017-11-28
RADIANT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented product has been found effective at promoting tissue growth on damaged areas such as burns by stimulating cells called histocytes that help them recover from damage caused during surgery procedures. It can also promote faster recovery time after surgeries without causing any negative side-effects like inflammations due to its ability to stop bleeding quickly while it stops getting better with each new procedure.

Problems solved by technology

Technological Problem addressed in this patents relating to improving the effectiveness of skin care products like those described earlier involves developing safer alternatives than current methods based on sutures or adhesive dressings. Additionally, there have also been studies exploring how certain agents could prevent unwanted reactions during wounding processes while promoting wound repair mechanisms.

Method used

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  • Cosmetic composition for skin regeneration and pharmaceutical composition for wound treatment, containing sinapic acid, which is marker component of cynanchum atratum extracts, or cynanchum atratum extracts containing same
  • Cosmetic composition for skin regeneration and pharmaceutical composition for wound treatment, containing sinapic acid, which is marker component of cynanchum atratum extracts, or cynanchum atratum extracts containing same
  • Cosmetic composition for skin regeneration and pharmaceutical composition for wound treatment, containing sinapic acid, which is marker component of cynanchum atratum extracts, or cynanchum atratum extracts containing same

Examples

Experimental program
Comparison scheme
Effect test

preparation example 1

[0046] (Preparation Example 1: Preparation of Bai Wei Extract)

[0047] Baiwei extract was prepared in this preparation example.

[0048] Buy the dried whole plant of Baiwei and use a pulverizer to prepare it into powder. The powdered Baiwei was extracted using 70% ethanol, 99.5% dichloromethane, and 99% ethylacetate as extraction solvents. At this time, 5-20 ml of each extraction solvent is used per 1 g of the dry weight of the Bai Wei powder. For the extraction, the extraction was carried out at room temperature for 3 hours. After extraction, filter paper is used to recover the extract. The recovered extracts were respectively concentrated using a rotary vacuum concentrator, and then used in the following tests.

experiment example 1

[0049] (Experimental Example 1: Determination of Cytotoxicity and Cell Growth of Bai Wei Extract)

[0050] In this experimental example, "MTT assay" was performed on the Baiwei extract prepared in Preparation Example 1 above to confirm cytotoxicity.

[0051] Human-derived keratinocytes (HaCaT) were inoculated and cultured at a concentration of 1×10 5 cells / ml in a 96-well plate. After that, each of the extracts of Baiwei prepared in the above-mentioned Preparation Example 1 was diluted to the concentrations in the following Table 1, added to the above-mentioned well plates and further cultured for 24, 48, and 72 hours. At this time, the MTT reagent was used to evaluate the survival rate and growth rate of the cells, and the negative control group in which the sample was not treated was used as the control group.

[0052] Table 1

[0053]

[0054] Experimental results, in 70% ethanol Bai Wei extract 25 treatment g / ml, 50 treatment g / ml, 99.5% dichloromethane 1 treatment g / ...

experiment example 2

[0055] (Experimental example 2: Determination of cell migration ability of Bai Wei extract)

[0056] In this experiment, the movement of keratinocytes was confirmed as an important mechanism during the above-mentioned treatment process.

[0057] In a 24-well plate, keratinocytes (HaCaT) were inoculated and cultured at a concentration of 1×10 5 cells / ml, and the cells were extracted using a pipette tip of 200 treatment liters. Afterwards, the 70% ethanol Baiwei extract that did not show cytotoxicity in the above-mentioned Experimental Example 1 was added at 25 g / ml, 50 g / ml, 99.5% dichloromethane at 5 g / ml, and 99% ethyl acetate Ester 5 was treated at g / ml to measure cell motility at 72 hr. In this case, epidermal growth factors with high cell mobility were used in the control group.

[0058] As a result of the experiment, compared with the epidermal growth factor as the comparison group, the Baiwei extract has excellent cell mobility, and it presents the most excellent cell ...

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Abstract

The present invention relates to a cosmetic composition for skin regeneration and a pharmaceutical composition for wound treatment, the compositions containing, as an active ingredient, sinapic acid or Cynanchum atratum extracts containing the same. The cosmetic composition for skin regeneration and the pharmaceutical composition for wound treatment of the present invention, the compositions containing sinapic acid or Cynanchum atratum extracts containing the same, control occurrence of inflammation on the skin and simultaneously exhibit excellent cellular reproduction and wound treatment effects.

Description

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Claims

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Application Information

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Owner RADIANT
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