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A Dual-Channel Fluorescence Imaging Detects Trace Cu in Viable Cancer Cells 2+ and hg 2+ Methods

A fluorescence imaging, dual-channel technology, applied in fluorescence/phosphorescence, measurement devices, material analysis through optical means, etc., can solve the problems of low detection efficiency, inability to realize dual-channel imaging detection at the same time, and high detection cost

Active Publication Date: 2020-02-18
GUIZHOU UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] Therefore, the existing technology probes cannot realize dual-channel imaging detection of copper and mercury ions in living cells at the same time, and the detection efficiency is low and the detection cost is high.

Method used

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  • A Dual-Channel Fluorescence Imaging Detects Trace Cu in Viable Cancer Cells <sup>2+</sup> and hg <sup>2+</sup> Methods
  • A Dual-Channel Fluorescence Imaging Detects Trace Cu in Viable Cancer Cells <sup>2+</sup> and hg <sup>2+</sup> Methods
  • A Dual-Channel Fluorescence Imaging Detects Trace Cu in Viable Cancer Cells <sup>2+</sup> and hg <sup>2+</sup> Methods

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Embodiment 1

[0053] 1. The synthetic route of the probe:

[0054]

[0055] The specific preparation method of the probe:

[0056] 1) Synthesis of intermediates:

[0057] in N 2 Under protection, weigh tris(2-aminoethyl)amine (4.00g, 27.36mmol) in a 100ml three-neck flask, weigh Rhodamine B (1.64g, 3.42mmol) and dissolve it in 40mL of absolute ethanol, add dropwise The three-necked flask was refluxed for 36 hours after the addition was completed, and the ethanol was distilled off under reduced pressure, extracted with dichloromethane (3×100mL), the organic phase was dried overnight with anhydrous sodium sulfate, and the solvent was evaporated to obtain a red viscous substance. Chromatographic separation, the eluent is methanol / chloroform / triethylamine with a volume ratio of 9 / 1 / 1, N 2 Under protection, take 27.36mmol of tris(2-aminoethyl)amine in a 100ml three-necked bottle, measure 20mL of absolute ethanol and stir to reflux, then take 2-5mmol of Rhodamine B and dissolve it in 40mL o...

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Abstract

The invention discloses a method of detecting trace Cu<2+> and Hg<2+> in active cancer cells by two-channel fluorescence imaging. The method comprises the following steps: by taking tri-[2,2'-di(7-nitryl benzo-2-oxa-1,4-diazole)-4-amino ethyl-2''-rhodamine formamido ethyl]amine as a fluorescence imaging probe, detecting trace Cu<2+> and Hg<2+> in active cancer cells by two-channel fluorescence imaging; then incubating the active cells through the probe; then incubating the incubated cells of the probe with Cu<2+> and Hg<2+>, separately; and detecting clear green and red fluorescent cell images by means of a fluorescent inverted microscope. The method disclosed by the invention can achieve two-channel imaging detection of copper and mercury ions in the living cells, and is high in detection efficiency and low in detection cost.

Description

technical field [0001] The invention relates to a method for detecting trace ions in living cells by dual-channel fluorescence imaging, in particular to a method for detecting trace Cu in active cancer cells by dual-channel fluorescence imaging 2+ and Hg 2+ Methods. Background technique [0002] Some heavy metals and transition metal elements have very important physiological roles and functions because of their participation in cell growth and development, gene transcription, neurotransmission, etc. It will cause obvious damage to the shape and structure. Therefore, to study the influence of various ions on organisms, a very necessary method is the online, visualized and real-time detection of active intracellular ions. [0003] Copper plays important functions in organisms, such as oxidizing iron before being transported to tissues and organs, and directly participating in iron metabolism through ceruloplasmin. Copper is present in numerous proteins and acts as an auxi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64
CPCG01N21/6428G01N2021/6439
Inventor 曾晞方浚安廖贤牟兰
Owner GUIZHOU UNIV
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