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Method for establishing a reduced mobility - mass spectra database for quickly screening drugs and application

A technology of reducing mobility and establishing methods, which is applied in the field of drug detection, can solve problems such as high cost, no screening process, and radioactivity, and achieve the effects of rapid response, shortened analysis cycle, and simple operation

Inactive Publication Date: 2017-12-12
SHANGHAI CRIMINAL SCI TECH RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0013] Seongshin Gwak et al. have developed the combination of IMS and DART-Q-TOF-MS to quickly screen 35 new psychoactive substances (NPS) in in vitro samples. However, the ion mobility spectrum uses a nickel source ion source, which is radioactive and It uses secondary mass spectrometry, which is expensive and has not established a usable screening process

Method used

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  • Method for establishing a reduced mobility - mass spectra database for quickly screening drugs and application
  • Method for establishing a reduced mobility - mass spectra database for quickly screening drugs and application
  • Method for establishing a reduced mobility - mass spectra database for quickly screening drugs and application

Examples

Experimental program
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Effect test

Embodiment 1

[0080] Example 1 Contains the establishment method of the reduced mobility-mass spectrum database of 53 kinds of drugs

[0081] The present embodiment detects respectively the reduced mobility and the mass spectrum database of 53 kinds of drug standard samples (abbreviated as samples in this implementation), and investigates its intraday precision and interday precision, including the following steps:

[0082] 1. Prepare the solution

[0083] Stock solution: prepare the standard stock solution of the sample with methanol or acetonitrile, the concentration is 1mg / mL or 0.5mg / mL or 0.1mg / mL.

[0084] Working solution: Dilute the stock solution of the above sample to a concentration of 0.02 μg / mL, 0.05 μg / mL, 0.10 μg / mL, 0.20 μg / mL, 0.50 μg / mL, 1.0 μg / mL, 2.0 μg / mL.

[0085] Calibration solution: prepare 0.1mg / mL cocaine; use various specifications of PEG to prepare polyethylene glycol with an average relative molecular mass of 600 and dilute to 1‰ with methanol.

[0086] All t...

Embodiment 2

[0111] Example 2 A detection method for rapid screening of drugs using the reduced mobility-mass spectrum database

[0112] In this example, the same IMS conditions and DART-TOF-MS mass spectrometer conditions as in Example 1 are used in combination with the above screening process, and a certain collected object is taken as an example to perform screening to confirm its type.

[0113] The usual procedure for screening is:

[0114] (1) Prepare the collected sample material into a 1 mg / mL solution, so that the concentration of the sample to be tested is 0.02-2 μg / mL, and perform IMS detection and DART-TOF-MS detection.

[0115] (2) In IMS detection, use the obtained drift time to calculate the reduced mobility k 0 ’, see image 3 , compare the reduced mobility with the database, find out the substances with a deviation (that is, the alarm threshold) between ±0.03, determine the range of suspicious substances, and initially screen out k 0 The substances with values ​​between ...

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Abstract

Provided are a method for establishing a reduced mobility - mass spectra database for quickly screening drugs and application. An ion mobility spectroscopy is combined with real-time direct analysis of ion source time-of-flight mass spectrometry, and a database is established and includes reduced mobility (k0) obtained by the ion mobility spectroscopy and also includes a mass number of a quasi-molecular ion peak generated by real-time direct analysis of the ion source time-of-flight mass spectrometry, as well as a mass number of a fragment ion peak generated by a collision induced ionization mode, the obtained database is utilized to perform high-throughput drug screening, sample processing is simple, no chromatographic separation is required, test results are accurate, reliable and easy to operate, and fake positive results are avoided. The established rapid screening method can be used for the rapid detection of multiple drugs with the detection limit range of 0.02-2 micrograms / mL.

Description

technical field [0001] The invention belongs to the technical field of drug detection, and in particular relates to a method for establishing a reduced mobility-mass spectrum database for fast screening of drugs and its application. Background technique [0002] Drug crime is one of the most harmful crimes to society, and the abuse of drugs and poisons is also a social problem that is widely concerned. Over the past decade, a thorny problem has emerged around the world in the form of the manufacture, distribution and abuse of drugs, including traditional morphine, heroin, cannabis, amphetamines and new psychoactive substances. [0003] Drug poison analysis is one of the important links in cracking down on drug-related crimes. As the number of drug-related cases continues to rise, it has increased the heavy burden on law enforcement agencies and intensified the demand for rapid drug screening technology. Therefore, the establishment of rapid drug screening The investigation ...

Claims

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Application Information

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IPC IPC(8): G01N27/62
CPCG01N27/622
Inventor 张玉荣连茹吕小宝吴忠平梁晨倪春芳汪蓉
Owner SHANGHAI CRIMINAL SCI TECH RES INST
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